Safety of intravitreally injected ciprofloxacin in phakic rabbit eyes.
- Author:
Sang Ha KIM
1
;
Jae Hong KIM
;
Kyung Shin CHO
;
Jung Sik KWAK
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: intravitreal ciprofloxacin; electroretinogram; Naka-Rushton equation parameters; electron microscory; retinal toxicity
- MeSH: Animals; Ciprofloxacin/administration & dosage/*toxicity; Dose-Response Relationship, Drug; Electroretinography/drug effects; Injections; Lens, Crystalline; Photic Stimulation; Rabbits; Retina/*drug effects/pathology/physiopathology; Rod Cell Outer Segment/drug effects/pathology; Vitreous Body
- From:Korean Journal of Ophthalmology 1995;9(1):12-18
- CountryRepublic of Korea
- Language:English
- Abstract: This study was designed to determine the maximal safe drug concentration of intravitreal ciprofloxacin in phakic rabbit eyes. Twenty-two eyes of New Zealand pigmented rabbits received midvitreal ciprofloxacin of 100, 200, 400, 600 or 800 microgram in BSS Plus, or BSS Plus only. Retinal toxicity was dose-dependent as determined with electroretinography, light microscopy, and transmission electron microscopy. At a dose of greater than 400 microgram, disorganization of the outer segments was a main pathological finding in transmission electron microscopy. We evaluated retinal function by measuring the electroretinograms for a graded series of flash intensities and by fitting electroretinogram b-wave amplitudes to the Naka-Rushton equation. At a dose of greater than 600 microgram, Rmax was significantly decreased and log K was significantly increased. N-value tended to decrease. A decrease of b-wave amplitudes caused by retinal toxicity could be detected very sensitively with lower luminance stimuli. Determination of retinal toxicity with lower luminance electroretinography revealed a significant decrease of b-wave amplitudes at a dose of greater than 400 microgram. We concluded that a safe dose of intravitreal ciprofloxacin in phakic rabbit eyes was 200 microgram in phakic eyes.
