Expression of p53 and Matrix Metalloproteinase-2 Proteins in Colorectal Adenocarcinoma.
- Author:
Seong Jin CHO
;
Hwa Eun OH
;
Yang Seok CHAE
- Publication Type:Original Article
- Keywords:
Colorectal adenocarcinoma;
p53;
MMP-2;
Tumor invasion;
Metastasis
- MeSH:
Adenocarcinoma*;
Adenoma;
Antibodies;
Collagenases;
Colorectal Neoplasms;
Extracellular Matrix;
Genes, p53;
Humans;
Lymph Nodes;
Matrix Metalloproteinase 2*;
Neoplasm Metastasis;
Polyps
- From:Korean Journal of Pathology
2000;34(7):494-500
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The p53 gene is believed to play an important role through the mutation and overexpression in the progression of various human malignant tumors. The type IV collagenase (matrix metalloproteinase: MMP-2) initiates the degradation of the extracellular matrix, and consequently may play a role in the tumor invasion and metastasis. To investigate the correlation between clinicopathologic features of the colorectal adenocarcinomas and benign tumors and expression of p53 and MMP-2 proteins, we performed an immunohistochemical study on 40 colorectal adenocarcinomas, 20 adenomas and 20 hyperplastic polyps by using the antibodies to p53 and MMP-2 proteins. The positive expression rate of the p53 protein in adenocarcinomas was 62.5% and significantly higher than in benign tumors. The positive expression rate of the MMP-2 protein was 47.5% in adenocarcinomas, but there was no expression of MMP-2 protein in benign tumors. The difference in p53 and MMP-2 expression rates between malignant and non-malignant tumors was statistically significant. The positive expression rate of p53 protein in the non-metastatic and metastatic adenocarcinomas was 59.1 and 66.7%, respectively. The positive expression rate of MMP-2 protein in the non-metastatic and metastatic adencarcinomas was 45.5 and 50.0%, respectively. The correlation between several clinicopathologic features and expression of p53 and MMP-2 protein was not statistically significant, but the rate of positive MMP-2 immunoreactivity showed a statistically significant difference between Astler-Coller stage B1 C1 group and B2 C2 group of adenocarcinoma (p=0.0431). We concluded that the expression of p53 and MMP-2 protein contributes to the cancer development and MMP-2 may play a certain role in the invasiveness of the colorectal tumor. p53 and MMP-2 protein expression is not correlated with lymph node metastasis.
