Effects of low dose irradiation on the calcific nodule formation in MC3T3-E1 osteoblastic cell line.
- Author:
Kyung A KIM
1
;
Kwang Joon KOH
Author Information
1. Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bio Science, Chonbuk National University, Korea. radkoh@chonbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Radiation;
Low Dose;
Osteoblastic Cell Line;
Calcification
- MeSH:
Calcium;
Cell Line*;
Osteoblasts*
- From:Korean Journal of Oral and Maxillofacial Radiology
2004;34(3):137-144
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of low dose irradiation on the calcium content and calcific nodule formation of the MC3T3-E1 osteoblastic cell line. MATERIALS AND METHODS: Cells were irradiated with a single dose of 0.2, 0.4 and 0.6 Gy at a dose rate of 5.38 Gy/min using Cs-137 irradiator. After irradiation, the calcium content and calcific nodule formation were examined on the 1st, 2nd, 3rd and 4th week. RESULTS: We did not find any significant difference of total calcium content after irradiation of 0.2, 0.4 and 0.6 Gy when compared with the unirradiated control group. There was no significant difference of total calcium content between 0.2, 0.4 and 0.6 Gy irradiated groups. We found an increased tendency of the calcific nodule formation after irradiation of 0.2, 0.4 and 0.6 Gy when compared with the unirradiated control group without significant difference of calcific nodule formation between 0.2, 0.4 and 0.6 Gy irradiated groups. CONCLUSION: The results showed an increased tendency of the calcific nodule formation after low dose irradiation. However, this tendency did not increase with the increase of irradiation dose.
