Some Studies on the Non-Swelling Cornea.
- Author:
Kwang Woo CHOI
1
;
Dong Ho YOUN
Author Information
1. Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea.
- Publication Type:Original Article
- MeSH:
Cornea*;
Epithelium;
Formaldehyde;
Lysine;
Silica Gel;
Sodium Chloride;
Thimerosal
- From:Journal of the Korean Ophthalmological Society
1974;15(4):276-278
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Non-swelling cornea was prepared according to the method of Payrau. 1) Rabbit cornea without epithelium was treated with the vapor of 0.04% formalin at 4 degrees C for 48 hours, and then dehydrated with silica gel at room temperature for 48 hours. 2) Dehydrated cornea was soaked in the neutral solution of 0.5% L-lysine HCI for one hour, and in the neutral solution of 0.5% L-arginin HCl for one hour successively. 3) Non-swelling cornea was preserved in the desiccator with silica gel or in 0.02% merthiolate solution. Authors compared the hydration ratio of the non-swelling cornea with untreated cornea. Authors employed the chromotropic acid method to measure the amount of formaldehyde in the non-s-welling cornea and compared with the cornea treated only with formalin showed that: 1) Non-swelling cornea became hydrated within 30 minutes and remained constant thereafter in 0.9% saline solution at room temperature, whereas formalin treated cornea hydrated enormously within 3 hours. 2) The amount of formaldehyde in the non-swelling cornea was on the average 0.129 micro M/mg dry weight, and 3) The amount of formaldehyde in the formalin treated cornea was on the average 0.325 micro M/mg dry weight.
