Cedrol Enhances Extracellular Matrix Production in Dermal Fibroblasts in a MAPK-Dependent Manner.
- Author:
Mu Hyun JIN
1
;
Sun Gyoo PARK
;
Yul Lye HWANG
;
Min Ho LEE
;
Nam Ji JEONG
;
Seok Seon ROH
;
Young LEE
;
Chang Deok KIM
;
Jeung Hoon LEE
Author Information
- Publication Type:Original Article
- Keywords: Cedrol; Collagen; Fibroblasts
- MeSH: Blotting, Western; Collagen; Collagen Type I; Elastin; Enzyme-Linked Immunosorbent Assay; Extracellular Matrix; Fibroblasts; Humans; Phosphorylation; Phosphotransferases; Protein Kinases; Pterocarpus; Skin; Terpenes
- From:Annals of Dermatology 2012;24(1):16-21
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: The extracellular matrix (ECM) produced by dermal fibroblasts supports skin structure, and degradation and/or reduced production of ECM are the main causes of wrinkle formation. OBJECTIVE: The aim of this study was to identify the active ingredient that enhances ECM production in dermal fibroblasts. METHODS: Polarity-based fractionation was used to isolate the active ingredient from natural extracts, and the effects of cedrol (isolated from Pterocarpus indicusirginia) on ECM production in cultured human dermal fibroblasts was investigated by reverse transcription-polymerase chain reaction, enzyme linked immunosorbent assay, and Western blot analysis. RESULTS: Cedrol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen and elastin. Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was markedly increased by cedrol, indicating that enhanced ECM production is linked to activation of intracellular signaling cascades. CONCLUSION: These results indicate that cedrol stimulates ECM production, with possible applications to the maintenance of skin texture.
