Nuclear factor of activated T cells negatively regulates expression of the tumor necrosis factor receptor-related 2 gene in T cells.
10.3858/emm.2010.42.12.083
- Author:
Woon Ki KIM
1
;
Ok Ju SUL
;
Jung Sook KWAK
;
Hye Young HUR
;
Anne M LATOUR
;
Beverly H KOLLER
;
Byoung S KWON
;
Choon Soo JEONG
Author Information
1. Department of Biological Science and the Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Korea. csjeong@ulsan.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
gene expression regulation;
NFATC transcription factors;
promoter regions, genetic;
T lymphocytes;
tumor necrosis factor ligand superfamily member 14
- MeSH:
Animals;
Base Sequence;
CD4-Positive T-Lymphocytes/metabolism;
Cells, Cultured;
Down-Regulation;
Mice;
Mice, Inbred C57BL;
Molecular Sequence Data;
NFATC Transcription Factors/*physiology;
Receptors, Tumor Necrosis Factor, Member 14/*biosynthesis;
T-Lymphocytes/*metabolism
- From:Experimental & Molecular Medicine
2010;42(12):805-810
- CountryRepublic of Korea
- Language:English
-
Abstract:
Tumor necrosis factor receptor-related 2 (TR2, HVEM or TNFRSF-14) plays an important role in immune responses, however, the mechanisms regulating its expression are unclear. To understand the control of TR2 gene expression, we studied the upstream region of the gene. Gel supershift assays revealed inducible binding of nuclear factor of activated T cells (NFAT) to a putative NFAT site within the TR2 promoter. Furthermore, cotransfection of a dominant negative NFAT construct, or siRNA for NFAT, resulted in increased expression of a TR2 reporter gene. Our findings demonstrate that NFAT negatively regulates TR2 expression in activated T cells.
