Cytoprotective effect of polysaccharide isolated from different mushrooms against 7-ketocholesterol induced damage in mouse liver cell line (BNL CL. 2).
- Author:
Joo Shin KIM
1
;
Hau Yin CHUNG
;
Keun NA
Author Information
- Publication Type:Original Article
- Keywords: 7-ketocholesterol; cytotoxicity; mushroom; polysaccharide
- MeSH: Agaricales*; Agaricus; Agrocybe; Animals; Cell Line*; Cordyceps; Flammulina; Liver*; Mice*; Polysaccharides; Shiitake Mushrooms; Water
- From:Nutrition Research and Practice 2007;1(3):180-183
- CountryRepublic of Korea
- Language:English
- Abstract: Cytoprotective ability of polysaccharides isolated from different edible mushrooms was investigated on the 7-ketocholesterol-induced damaged cell line. Polysaccharide extracts from six different edible mushrooms-Flammulina velutipes, Peurotus ostreatus, Lentinus edodes, Agrocybe aegerita, Agaricus blazei, and Cordyceps militaris- were prepared by hot water extraction and alcohol precipitation. Cytoprotective ability was evaluated by measuring the viable cells of the normal embryonic liver cell line (BNL CL. 2) in the presence of 7-ketocholesterol. At 80 microgram/mL of 7-ketocholesterol, cytotoxicity was very high with a loss of 98% of viable cells after 20 h of incubation. With the addition of 200 microgram/mL of each polysaccharide isolate to the cell line containing 80 microgram/mL of 7-ketocholesterol, polysaccharide isolates from both Flammulina velutipes and Peurotus ostreatus could significantly inhibit the 7-ketochoelsterol-induced cytotoxicity in the cells. But other polysaccharide isolates were not effective in inhibiting cell damage caused by the oxLDL-induced cytotoxicity.
