A study on the chromosomal aberrations in Korean intrahepatic cholangiocarcinomas with comparative genomic hybridization.
- Author:
Seong Ho CHOI
1
;
Tae Sung SOHN
;
Jae Won JOH
;
Sung Joo KIM
;
Jin Seok HEO
;
Yong Il KIM
;
Kwang Soo LEE
;
Youl Hee CHO
Author Information
1. Department of General Surgery, College of Medicine, Sungkyunkwan University, Samsung Medical Center, Korea.
- Publication Type:Original Article
- Keywords:
Comparative genomic hybridization;
Intrahepatic cholangiocarcnoma
- MeSH:
Arm;
Cholangiocarcinoma*;
Chromosome Aberrations*;
Chromosomes, Human, Pair 19;
Comparative Genomic Hybridization*;
DNA;
Genome;
Mass Screening
- From:Korean Journal of Hepato-Biliary-Pancreatic Surgery
1999;3(2):77-88
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
AIMS AND METHOD: Comparative genomic hybridization serves as a screening test for regions of copy number changes in tumor genomes. I have applied the technique to map DNA losses and gains in 13 cases of frozen cholangiocarcinomas. RESULTS: All of the 13 cases showed genetic alterations. Loss of short arm of chromosome 19 (92%) was the most common changes observed. 22q(62%), 1p(54%), 17p(54%) and 19q(54%) also showed nonrandom patterns of genomic losses with high frequencies. Among the genomic gains, 13q was revealed as the most common site (69%), and 8q (46%) and 12q (46%) also showed relatively high frequencies of genomic gains. Genomic amplifications were detected on 5p13, 10q21.1 and 18q11.3 in 3 different cases, respectively. CONCLUSION: This study represents the first analysis of intrahepatic cholangiocarcinomas by CGH, and it confirms the presence of nonrandom genetic changes occur in the pathogenesis of cholangiocarcinomas. These findings should lead to the characterization of new loci involved in cholangiocarcinoma pathogenesis.
