Stage-specific embryonic antigen: determining expression in canine glioblastoma, melanoma, and mammary cancer cells.
10.4142/jvs.2017.18.1.101
- Author:
Weiming LIN
1
;
Jaime F MODIANO
;
Daisuke ITO
Author Information
1. Department of Veterinary Clinical Sciences and Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA. modiano@umn.edu wmlin925@126.com
- Publication Type:Brief Communication
- Keywords:
canine cancer;
flow cytometry;
magnetic-activated cell sorting;
stage-specific embryonic antigens
- MeSH:
Antigens, CD15;
Breast Neoplasms*;
Cell Line;
Flow Cytometry;
Glioblastoma*;
Hemangiosarcoma;
Lymphoma;
Melanoma*;
Osteosarcoma;
Stage-Specific Embryonic Antigens*
- From:Journal of Veterinary Science
2017;18(1):101-104
- CountryRepublic of Korea
- Language:English
-
Abstract:
The expression of stage-specific embryonic antigens (SSEAs) was determined in several types of canine cancer cells. Flow cytometry showed SSEA-1 expression in glioblastoma, melanoma, and mammary cancer cells, although none expressed SSEA-3 or SSEA-4. Expression of SSEA-1 was not detected in lymphoma, osteosarcoma, or hemangiosarcoma cell lines. Relatively stable SSEA-1 expression was observed between 24 and 72 h of culture. After 8 days in culture, sorted SSEA-1⁻ and SSEA-1⁺ cells re-established SSEA-1 expression to levels comparable to those observed in unsorted cells. Our results document, for the first time, the expression of SSEA-1 in several canine cancer cell lines.
