The Efficacy of alpha-lipoic Acid on the Endotoxin-induced Acute Lung Injury.
10.4046/trd.2007.62.2.105
- Author:
Jin Won HUH
1
;
Sang Bum HONG
;
Mi Jung KIM
;
Chae Man LIM
;
Younsuck KOH
Author Information
1. Department of Internal Medicine, Ilsan Paik Hospital, Inje University, Goyang, Korea,
- Publication Type:Original Article
- Keywords:
alpha-lipoic acid;
oxidative stress;
acute lung injury;
antioxidant
- MeSH:
Acute Lung Injury*;
Animals;
Bronchoalveolar Lavage;
Interleukin-6;
Lung;
Models, Animal;
Neutrophil Infiltration;
Neutrophils;
Oxidative Stress;
Peroxidase;
Rats;
Rats, Sprague-Dawley;
Thioctic Acid*;
Trachea;
Tumor Necrosis Factor-alpha
- From:Tuberculosis and Respiratory Diseases
2007;62(2):105-112
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Oxidative stress may play an important role in the pathogenesis of endotoxin-induced acute lung injury (ALI). This study evaluated the therapeutic effect of alpha-lipoic acid, a nonenzymatic antioxidant, in a rat model of lipopolysaccharide (LPS) induced ALI. MATERIALS AND METHODS: ALI was induced in Sprague-Dawley rats by instilling LPS (E.coli, 3mg/Kg) into the trachea. The rats were classified into the control, control+alpha-lipoic acid, LPS, and LPS+alpha-lipoic acid groups.The lung lavage neutrophil count, cytokine-induced neutrophil chemoattractant (CINC), lung myeloperoxidase (MPO), and cytokine concentrations (TNF-alpha, IL-1beta, IL-6 and IL-10) were measured at 2 h and 6 h after LPS administration. RESULTS: The total cell and neutrophil counts of the LPS+alpha-lipoic acid groups were significantly lower than the LPS groups. The protein concentration in the BAL fluid was similar in the LPS groups and LPS+alpha-lipoic acid groups. The TNF-alpha, IL-1beta, and IL-6 concentrations in the BAL fluid were not decreased by the alpha-lipoic acid treatment in the LPS treated rats. CONCLUSIONS: Although alpha-lipoic acid decreased the level of LPS-induced neutrophil infiltration into the lung, it could not attenuate the LPS-induced ALI at the dose administered in this study.
