Postnatal Treadmill Exercise Alleviates Prenatal Stress-Induced Anxiety in Offspring Rats by Enhancing Cell Proliferation Through 5-Hydroxytryptamine 1A Receptor Activation.
- Author:
Sam Jun LEE
1
;
Tae Woon KIM
;
Hun Kyung PARK
;
Sangyun YOON
;
Ann Hee YOU
;
Eun Jin MOON
;
Dong Hoon SHIN
;
Hanjin CHO
Author Information
1. Department of Physical Education, College of Health, Welfare, and Education, Tong Myong University, Busan, Korea.
- Publication Type:Original Article
- Keywords:
Prenatal Exposure Delayed Effects;
Exercise Test;
Anxiety;
Cell Proliferation;
Serotonin
- MeSH:
Animals;
Anxiety*;
Blotting, Western;
Brain-Derived Neurotrophic Factor;
Bromodeoxyuridine;
Cell Proliferation*;
Dentate Gyrus;
Dogs;
Dorsal Raphe Nucleus;
Exercise Test;
Hippocampus;
Immunohistochemistry;
Pregnancy;
Prenatal Exposure Delayed Effects;
Protein-Tyrosine Kinases;
Rats*;
Receptor, Serotonin, 5-HT1A*;
Risk Factors;
Serotonin*
- From:International Neurourology Journal
2016;20(Suppl 1):S57-S64
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Stress during pregnancy is a risk factor for the development of anxiety-related disorders in offspring later in life. The effects of treadmill exercise on anxiety-like behaviors and hippocampal cell proliferation were investigated using rats exposed to prenatal stress. METHODS: Exposure of pregnant rats to a hunting dog in an enclosed room was used to induce stress. Anxiety-like behaviors of offspring were evaluated using the elevated plus maze test. Immunohistochemistry for the detection of 5-bromo-2'-deoxyuridine and doublecortin (DCX) in the hippocampal dentate gyrus and 5-hydroxytryptamine 1A receptors (5-HT(1A)) in the dorsal raphe was conducted. Brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB) levels in the hippocampus were evaluated by western blot analysis. RESULTS: Offspring of maternal rats exposed to stress during pregnancy showed anxiety-like behaviors. Offspring also showed reduced expression of BDNF, TrkB, and DCX in the dentate gyrus, decreased cell proliferation in the hippocampus, and reduced 5-HT(1A) expression in the dorsal raphe. Postnatal treadmill exercise by offspring, but not maternal exercise during pregnancy, enhanced cell proliferation and expression of these proteins. CONCLUSIONS: Postnatal treadmill exercise ameliorated anxiety-like behaviors in offspring of stressed pregnant rats, and the alleviating effect of exercise on these behaviors is hypothesized to result from enhancement of cell proliferation through 5-HT(1A) activation in offspring rats.
