Comparative Ki-67 Expression and Apoptosis in the Odontogenic Keratocyst Associated with or without an Impacted Tooth in Addition to Unilocular and Multilocular Varieties.
10.3349/ymj.2003.44.5.841
- Author:
Do Kyung KIM
1
;
Sang Gun AHN
;
Jin KIM
;
Jung Hoon YOON
Author Information
1. Department of Oral Pathology, School of Dentistry, Chosun University, Seosuk-dong Dong-gu, Gwangju, Korea. jhyoon@chosun.ac.kr
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Odontogenic keratocyst;
impacted tooth;
proliferative activity;
apoptosis
- MeSH:
Adult;
*Apoptosis;
Cell Division;
Comparative Study;
Female;
Human;
Immunohistochemistry;
In Situ Nick-End Labeling;
Ki-67 Antigen/*analysis;
Male;
Odontogenic Cysts/*pathology;
Proliferating Cell Nuclear Antigen/analysis;
Support, Non-U.S. Gov't;
Tooth, Impacted/*pathology
- From:Yonsei Medical Journal
2003;44(5):841-846
- CountryRepublic of Korea
- Language:English
-
Abstract:
It is not known whether the presence of an impacted tooth or the radiographic types in an odontogenic keratocyst (OKC) change the clinical biologic behavior and therapeutic approaches. This study evaluated the comparative proliferative activity and apoptosis in OKC associated with or without an impacted tooth, as well as between the unilocular and multilocular OKC varieties. Immunohistochemical expression of Ki-67 as a proliferation marker and the apoptotic reactions were assessed by the TUNEL method for 32 cases of OKC (OKC with impacted tooth, n=16; OKC without impacted tooth, n=16) and 10 cases of dentigerous cyst (DC). OKC showed a greater proliferative potential and more apoptotic reactions than DC. In particular, OKC contained proliferating and apoptotic cells situated predominantly in the suprabasal and superficial layers, respectively. However, no significant difference was found between OKC associated with or without impacted tooth, or between the unilocular and multilocular OKC varieties, in terms of proliferative activity or apoptosis. In conclusion, OKC is characterized by an increase in both cell proliferation and apoptosis, suggesting a unique proliferative and differentiation process. It is believed that incomplete removal or other contributing factors, rather than intrinsic growth or apoptosis, may be the main reasons for the aggressive biologic behavior or recurrence in multilocular OKC.
