Enhanced skin wound healing by a sustained release of growth factors contained in platelet-rich plasma.
10.3858/emm.2011.43.11.070
- Author:
Hee Seok YANG
1
;
Jaehoon SHIN
;
Suk Ho BHANG
;
Jung Youn SHIN
;
Jooyeon PARK
;
Gun Il IM
;
Chang Sung KIM
;
Byung Soo KIM
Author Information
1. School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea. byungskim@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
angiogenesis inducing agents;
endothelial growth factors;
fibrin;
fibroblast growth factor 2;
heparin;
neovascularization, physiologic;
platelet-derived growth factor;
platelet-rich plasma;
wound healing
- MeSH:
Animals;
Blotting, Western;
*Cell Proliferation;
Dermis/cytology/metabolism;
Female;
Fibrin/*metabolism;
Fibroblast Growth Factor 2/genetics/metabolism;
Heparin/metabolism;
Immunoenzyme Techniques;
Intercellular Signaling Peptides and Proteins/*secretion;
Mice;
Mice, Inbred BALB C;
Platelet-Rich Plasma/*metabolism;
Proto-Oncogene Proteins c-sis/genetics/metabolism;
RNA, Messenger/genetics;
Real-Time Polymerase Chain Reaction;
Regeneration;
Skin/*cytology/*metabolism;
Vascular Endothelial Growth Factor A/genetics/metabolism;
Wound Healing/*physiology
- From:Experimental & Molecular Medicine
2011;43(11):622-629
- CountryRepublic of Korea
- Language:English
-
Abstract:
Platelet-rich plasma (PRP) contains growth factors that promote tissue regeneration. Previously, we showed that heparin-conjugated fibrin (HCF) exerts the sustained release of growth factors with affinity for heparin. Here, we hypothesize that treatment of skin wound with a mixture of PRP and HCF exerts sustained release of several growth factors contained in PRP and promotes skin wound healing. The release of fibroblast growth factor 2, platelet-derived growth factor-BB, and vascular endothelial growth factor contained in PRP from HCF was sustained for a longer period than those from PRP, calcium-activated PRP (C-PRP), or a mixture of fibrin and PRP (F-PRP). Treatment of full-thickness skin wounds in mice with HCF-PRP resulted in much faster wound closure as well as dermal and epidermal regeneration at day 12 compared to treatment with either C-PRP or F-PRP. Enhanced skin regeneration observed in HCF-PRP group may have been at least partially due to enhanced angiogenesis in the wound beds. Therefore, this method could be useful for skin wound treatment.
