Protective Effect of an Isoflavone, Tectorigenin, Against Oxidative Stress-induced Cell Death via Catalase Activation.
10.15430/JCP.2016.21.4.257
- Author:
Rui ZHANG
1
;
Mei Jing PIAO
;
Min Chang OH
;
Jeong Eon PARK
;
Kristina SHILNIKOVA
;
Yu Jin MOON
;
Dong Hyun KIM
;
Uhee JUNG
;
In Gyu KIM
;
Jin Won HYUN
Author Information
1. Department of Biochemistry, Jeju National University School of Medicine, Jeju, Korea. jinwonh@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
Isoflavones;
Tectorigenin;
Catalase;
Extracellular signal regulated kinases;
NF-kappa B
- MeSH:
Animals;
Blotting, Western;
Catalase*;
Cell Death*;
Cell Survival;
Cricetinae;
Cricetulus;
Electrophoretic Mobility Shift Assay;
Extracellular Signal-Regulated MAP Kinases;
Fibroblasts;
Hydrogen Peroxide;
Isoflavones;
Luciferases;
Lung;
NF-kappa B;
Oxidative Stress;
Phosphorylation;
Phosphotransferases;
Soybeans;
Transfection
- From:Journal of Cancer Prevention
2016;21(4):257-263
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Isoflavones are biologically active compounds that occur naturally in a variety of plants, with relatively high levels in soybean. Tectorigenin, an isoflavone, protects against hydrogen peroxide (H2O2)-induced cell damage. However, the underlying mechanism is unknown. METHODS: The MTT assay was performed to determine cell viability. Catalase activity was assessed by determining the amount of enzyme required to degrade 1 μM H2O2. Protein expression of catalase, phospho-extracellular signal-regulated kinase (ERK), IκB-α, and NF-κB were evaluated by Western blot analysis. A mobility shift assay was performed to assess the DNA-binding ability of NF-κB. Transient transfection and a NF-κB luciferase assay were performed to assess transcriptional activity. RESULTS: Tectorigenin reduced H2O2-induced death of Chinese hamster lung fibroblasts (V79-4). In addition, tectorigenin increased the activity and protein expression of catalase. Blockade of catalase activity attenuated the protective effect of tectorigenin against oxidative stress. Furthermore, tectorigenin enhanced phosphorylation of ERK and nuclear expression of NF-κB, while inhibition of ERK and NF-κB attenuated the protective effect of tectorigenin against oxidative stress. CONCLUSIONS: Tectorigenin protects cells against oxidative damage by activating catalase and modulating the ERK and NF-κB signaling pathway.
