The Effect of Sex on the Azoxymethane/Dextran Sulfate Sodium-treated Mice Model of Colon Cancer.
10.15430/JCP.2016.21.4.271
- Author:
Sun Min LEE
1
;
Nayoung KIM
;
Hee Jin SON
;
Ji Hyun PARK
;
Ryoung Hee NAM
;
Min Hee HAM
;
Daeun CHOI
;
Sung Hwa SOHN
;
Eun SHIN
;
Young Jae HWANG
;
Jihee SUNG
;
Dong Ho LEE
;
Ha Na LEE
Author Information
1. Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea. nayoungkim49@empas.com
- Publication Type:Original Article
- Keywords:
Sex;
Disease models;
animal;
Colitis;
Colonic neoplasms
- MeSH:
Animals;
Carcinogenesis;
Colitis;
Colon*;
Colonic Neoplasms*;
Female;
Humans;
Interleukins;
Male;
Mice*;
Mice, Inbred ICR;
Models, Animal;
Peroxidase;
Sodium
- From:Journal of Cancer Prevention
2016;21(4):271-278
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The colitis-associated cancer exhibits different characteristics according to sex in the initiation and progression of the tumors. The aim of this study was to investigate the sex-associated difference in the azoxymethane/dextran sulfate sodium (AOM/DSS)-induced colitis-associated cancer model. METHODS: The AOM/DSS ICR mouse model was established to compare male with female, and then the severity of colitis-associated carcinogenesis was examined macroscopically and histologically regarding the number, size, and location of tumors. Subsequently, levels of colonic mucosal cytokine, interleukin (IL)-1β and myeloperoxidase (MPO) were assessed. RESULTS: At the 16th week, the tumor multiplicity and the pro-inflammatory factors differed according to sex. The total tumor number was significantly higher in male (P = 0.020) and the number of large tumors (diameter > 2 mm) was higher in male (P = 0.026). In male, the tumors located more in distal colon (P = 0.001). MPO was significantly higher in AOM/DSS-treated male mice compared to the control group (P = 0.003), whereas the corresponding female group showed no significant change (P = 0.086). Colonic IL-1β level significantly increased in AOM/DSS groups compared to control groups both in male and female (male, P = 0.014; female, P = 0.005). It was higher in male group; however, there was no statistical significance (P = 0.226). CONCLUSIONS: In AOM/DSS murine model, colitis-associated colon tumorigenesis are induced more severely in male mice than female probably by way of inflammatory mediators such as IL-1β and MPO. The sex-related differences at the animal model of colon cancer suggest the importance of approach to disease with sex-specific medicine in human.
