In vitro Protective Effects of Glehnia Littoralis on Alpha-amanitin Induced Hepatotoxicity.
- Author:
Bo Hyun KIM
1
;
Kyung Hoon SUN
;
Sun Pyo KIM
;
Yongjin PARK
Author Information
- Publication Type:Original Article
- Keywords: Alpha-amanitin; Antioxidant; Glehnia littoralis
- MeSH: Alpha-Amanitin*; Amanita; Antioxidants; Antipyretics; Apiaceae*; Calcium Channels; Carcinoma, Hepatocellular; Cell Line; Cell Survival; Cough; Coumarins; Fungi; Hep G2 Cells; Humans; In Vitro Techniques*; Methods; Neuralgia; Oxidative Stress; Paralysis; Poisoning; Stroke
- From:Journal of The Korean Society of Clinical Toxicology 2017;15(2):107-115
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: Glehnia littoralis has been used to treat ischemic stroke, phlegm, cough, systemic paralysis, antipyretics and neuralgia. The pharmacological mechanisms of Glehnia littoralis include calcium channel block, coumarin derivatives, anticoagulation, anti-convulsive effect, as well as anti-oxidant and anti-inflammatory effects. Alpha-amanitin (α-amanitin) is a major toxin from extremely poisonous Amanita fungi. Oxidative stress, which may contribute to severe hepatotoxicity was induced by α-amanitin. The aim of this study was to investigate whether Glehnia littoralis ethyl acetate extract (GLEA) has the protective antioxidant effects on α-amanitin -induced hepatotoxicity. METHODS: Human hepatoma cell line HepG2 cells were pretreated in the presence or absence of GLEA (50, 100 and 200µg/ml) for 4 hours, then exposed to 60µmol/L of α-amanitin for an additional 4 hours. Cell viability was evaluated using the MTT method. AST, ALT, and LDH production in a culture medium and intracellular MDA, GSH, and SOD levels were determined. RESULTS: GLEA (50, 100 and 200µg/ml) significantly increased the relative cell viability by 7.11, 9.87, and 14.39%, respectively, and reduced the level of ALT by 10.39%, 34.27%, and 52.14%, AST by 9.89%, 15.16%, and 32.84%, as well as LDH by 15.86%, 22.98%, and 24.32% in culture medium, respectively. GLEA could also remarkably decrease the level of MDA and increase the content of GSH and SOD in the HepG2 cells. CONCLUSION: In the in vitro model, Glehnia littoralis was effective in limiting hepatic injury after α-amanitin poisoning. Its antioxidant effect is attenuated by antidotal therapy.
