The Modulation of IL-12 by Phosphodiesterase IV Inhibitor and Subsequent Changes of Th-1, Th-2 Cytokines in Human Endometrial Tissue.
- Author:
Won Il PARK
1
;
Eun Kyong KIM
;
Duck Sung KO
;
Seo You HONG
;
Joong Yol NA
;
Jung Hwan SHIN
;
Dae Woon KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Eulji University, Korea.
- Publication Type:Original Article
- Keywords:
Rolipram;
Decidua;
IL-12;
Th1 cytokine;
Th2 cytokine
- MeSH:
Abortion, Missed;
Blotting, Western;
Cyclic Nucleotide Phosphodiesterases, Type 4*;
Cytokines*;
Decidua;
Dilatation;
Female;
Humans*;
Immunohistochemistry;
Interleukin-10;
Interleukin-12 Subunit p35;
Interleukin-12 Subunit p40;
Interleukin-12*;
Pregnancy;
Pregnant Women;
RNA, Messenger;
Rolipram;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Obstetrics and Gynecology
2003;46(8):1500-1509
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To assess the capability of phosphodiesterase type IV inhibitor (rolipram) to suppress IL-12 in human decidua and the subsequent changes of Th-2 cytokine (IL-10) and Th-1 cytokine (TNF-alpha). METHODS: Decidual tissues of 10 first-trimester pregnant women and 10 first-trimester pregnant women diagnosed as missed abortion were collected by dilatation and currettage. The decidual tissues were treated with rolipram for 6 hours. Protein and mRNA expression in the tissues were analysed by western blotting, immunohistochemistry and reverse transcription-polymerase chain reaction. RESULTS: Rolipram, in the concentration above 1 microgram/ml, could decrease the expression of IL-12p35 (control: 46.37+/-7.38, rolipram: 24.34+/-8.46) and IL-12p40 mRNA (control: 31.7+/-5.8, rolipram: 14.9+/-4.6) and protein (control: 52.4+/-8.9, rolipram: 40.9+/-12.1). However, the expression of IL-10 and TNF-alpha mRNA and protein did not changed by rolipram. There was no difference in the cytokine expression pattern between the decidual tissues of normal pregnancy and missed abortion. CONCLUSION: Rolipram, the phosphodiesterase type IV inhibitor, could induce the decrease of IL-12 in human decidua. In human decidual tissue, unlike other human tissues, the decrease of IL-12 by rolipram did not modulate other Th-1/Th-2 cytokines. Inability of IL-12 to modulate other Th-1/Th-2 cytokines might be related with unique cytokine network in human decidua rather than its small extent of decrease.
