Immunogenecity of Outer Membrane Proteins of vibro vulnificus.
- Author:
Hyun Su KIM
1
;
Wan Jae PARK
;
Na Gyong LEE
;
Jub Woo JU
;
Moon Soo HOE
;
Cho Rok JUNG
Author Information
1. Department of Microbiology, College of Natural Science, Pusan National University, Pusan, Korea. crjung@hyowon.pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Vibrio vulnificus;
Outer membrane protein;
ELISA;
Western blotting
- MeSH:
Blotting, Western;
Enzyme-Linked Immunosorbent Assay;
Membrane Proteins*;
Membranes*;
Sodium;
Vibrio vulnificus
- From:Journal of the Korean Society for Microbiology
1998;33(4):353-360
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To provide basic information on the pathogengenesis of Vibrio vulnificus infection and for the manufacture of effective vaccine, outer membrane proteins (OMPs) were extracted from V. vulnificus ATCC 27562 strain and analysed by 12% of sodium dodecyl sulfate-polyarylamide gel(SDS-PAGE). Major 36 kDa and 48 kDa, 46 kDa, 66 kDa and 24 kDa protein bands appeared on gel by Coomassie stain and determined by densitometer. Immunogenecity of these proteins was examined by enzyme-linked immunosorbant assay(ELISA) and western blotting with rabbit anti-V. vulnificus serum. OMPs reacted with this antiserum, and the major 36 kDa protein appeared most immunogenic.
