Effect of Three Dimensional Culture Using Alginate-Fibrin Beads on the Chondrogenic Differentiation of Rabbit's Perichondrial Cells.
- Author:
Won Joon YOO
1
;
In Ho CHOI
;
Chin Youb CHUNG
;
Tae Joon CHO
;
Cha Hui LEE
Author Information
1. Department of Orthopaedic Surgery, Seoul National University College of Medicine, Seoul, Korea. inhoc@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Alginate-fibrin bead;
Perichondrial cell;
Chondrogenic differentiation
- MeSH:
Aggrecans;
Cartilage;
Collagen;
Collagen Type II;
Fibrin;
Gene Expression;
Polymerase Chain Reaction;
Reverse Transcription;
Ribs;
Tibia
- From:Journal of Korean Orthopaedic Research Society
2003;6(1):1-6
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the usefulness of three dimensional culture using alginate-fibrin beads on the chondrogenic differentiation of rabbit's perichondrial cells. MATERIALS AND METHODS: Rib perichondrial cells from rabbit expanded by monolayer culture were cultured in monolayer, in alginate bead, and in alginate-fibrin beads. Reverse transcription polymerase chain reaction (RT-PCR) for type I, II, X collagen, and aggrecan was performed at 3 weeks after culture. At that time, we removed the alginate component from the alginate-fibrin bead. Then, the cell-fibrin beads were transplanted into the partial physeal defect of proximal tibia, and some beads were cultured on for additional 3 weeks for RT-PCR. Histologic examination was performed at 2, 4, 8, 12, and 16 weeks after operation. RESULTS: At 3 weeks, type II collagen gene expression was maintained regardless of the culture system used. However, at 6 weeks it was maintained only in three-dimensional culture system using alginate beads and fibrin beads. Histologic examination showed that the implanted perichondrial cell-fibrin beads formed small nest composed of chondrocyte-like cells and matrix. CONCLUSION: These results suggest that alginate-fibrin beads may be used as a biodegradable scaffold for cartilage engineering using perichondrial cells.
