LPS-induced Chemokine Gene Expression in Mesangial Cell.
- Author:
Hark RIM
;
Soo Jung YOON
;
Jong Wook PARK
- Publication Type:Original Article
- MeSH:
Chemokines;
Gene Expression*;
Inflammation;
Interferons;
Kidney;
Mesangial Cells*;
Renal Insufficiency;
RNA;
Sodium Salicylate
- From:Korean Journal of Nephrology
1999;18(6):847-855
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was designed to investigate the mole- cular mechanism of chemokine induction by lipopoly-saccharide(LPS) of E. coli. Chemokine gene expression was evaluated by the reverse transcriptase-polymerase chain reaction(RT-PCR) assay using RNAs isolated either from kidneys of LPS-injected inice or from the mesangial cells stimulated with LPS, IFN-r or TNF-a. LPS was shown to induce interferon gamma(IFN- 7 ) inducible protein 10 (IP-10) and monokine induced by interferon gamma (MIG) in kidney. IP-10 gene expression was induced by LPS and IFN-r, but MIG gene expression was induced by IFN-r in mesangial cell. Chemokines induced by LPS increased splenocyte migration. Sodium salicylate, wortmanin and piperazine blocked LPS mediated chemokine induction suggesting the activation of nuclear factor-a B pathway. It is concluded from this study that mesangial cells are the target of LPS in the renal failure resulting from the systemic infections. LPS induces chemokines directly and/or indirectly in the mesan- gial cells, and these chemokines may associated with renal inflammation.
