Effects of combined mechanical stimulation on the proliferation and differentiation of pre-osteoblasts.
10.3858/emm.2011.43.6.040
- Author:
Kyung Shin KANG
1
;
Seung Jae LEE
;
Haksue LEE
;
Wonkyu MOON
;
Dong Woo CHO
Author Information
1. Department of Mechanical Engineering, POSTECH, Pohang 790-751, Korea. dwcho@postech.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
bioreactors;
cell differentiation;
cyclic strain;
physical stimulation;
tissue engineering;
ultrasound
- MeSH:
Animals;
Bioreactors;
*Bone Regeneration;
Cell Differentiation;
Cell Line;
Cell Proliferation;
Lactic Acid/chemistry;
*Mechanical Processes;
Mechanotransduction, Cellular/physiology;
Mice;
Osteoblasts/cytology/*metabolism;
Polyesters/chemistry;
Polymers/chemistry;
Tissue Engineering/methods;
Tissue Scaffolds/chemistry/utilization
- From:Experimental & Molecular Medicine
2011;43(6):367-373
- CountryRepublic of Korea
- Language:English
-
Abstract:
We observed how combined mechanical stimuli affect the proliferation and differentiation of pre-osteoblasts. For this research, a bioreactor system was developed that can simultaneously stimulate cells with cyclic strain and ultrasound, each of which is known to effectively stimulate bone tissue regeneration. MC3T3-E1 pre-osteoblasts were chosen for bone tissue engineering due to their osteoblast-like characteristics. 3-D scaffolds were fabricated with polycaprolactone and poly-L-lactic acid using the salt leaching method. The cells were stimulated by the bioreactor with cyclic strain and ultrasound. The bioreactor was set at a frequency of 1.0 Hz and 10% strain for cyclic strain and 1.0 MHz and 30 mW/cm2 for ultrasound. Three experimental groups (ultrasound, cyclic strain, and combined stimulation) and a control group were examined. Each group was stimulated for 20 min/day. Mechanical stimuli did not affect MC3T3-E1 cell proliferation significantly up to 10 days when measured with the cell counting kit-8. However, gene expression analysis of collagen type-I, osteocalcin, RUNX2, and osterix revealed that the combined mechanical stimulation accelerated the matrix maturation of MC3T3-E1 cells. These results indicate that the combined mechanical stimulation can enhance the differentiation of pre-osteoblasts more efficiently than simple stimuli, in spite of no effect on cell proliferation.
