Isolation and Characterization of Human Muscle Cells.
- Author:
In Hung LEE
1
;
Kwan Pyo HUNG
;
Dong Yook KIM
;
Tae Hwan KIM
;
Jae Bum JUN
;
Sung See JUNG
;
Sang Cheol BAE
;
Dae Hyun YOO
;
Seong Yoon KIM
Author Information
1. Institute of Rheumatology, Department of Internal Medicine, Hanyang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Myoblats;
Culture;
Leu-19
- MeSH:
Arthroplasty, Replacement, Hip;
Biopsy;
Collagenases;
Humans*;
Muscle Cells*;
Muscle, Skeletal;
Myoblasts;
Trypsin
- From:The Journal of the Korean Rheumatism Association
1996;3(1):64-69
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To isolate and culture of human muscle cells by manipulating culture conditions. METHODS: Muscle samples were obtained during total hip replacement or bedside muscle biopsy. We isolated myoblasts from freshly obtained human muscle tissue by trypsin and collagenase digesion. RESULTS: Selective isolation of myoblasts was identified through histologic examination by light-microscope. We identified monoc]onal antibodies(Leu-19) as molecular markers in human skeletal muscle were expressed. CONCLUSIONS: This study demonstrated that it had been possible to culture morphologically and immunelogically identifiable myoblasts isolated from human skeletal muscle tissue.
