Preserved Hippocampal Glucose Metabolism on 18F-FDG PET after Transplantation of Human Umbilical Cord Blood-derived Mesenchymal Stem Cells in Chronic Epileptic Rats.
10.3346/jkms.2015.30.9.1232
- Author:
Ga Young PARK
1
;
Eun Mi LEE
;
Min Soo SEO
;
Yoo Jin SEO
;
Jungsu S OH
;
Woo Chan SON
;
Ki Soo KIM
;
Jae Seung KIM
;
Joong Koo KANG
;
Kyung Sun KANG
Author Information
1. Department of Neurology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea. jkkang@amc.seoul.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Lithium-pilocarpine;
Positron-emission Tomography;
Animal Model of Chronic Epilepsy;
Mesenchymal Stem Cell Transplantation;
Cell Therapy
- MeSH:
Animals;
Chronic Disease;
Cord Blood Stem Cell Transplantation/*methods;
Epilepsy, Temporal Lobe/*metabolism/pathology/*therapy;
Fluorodeoxyglucose F18/*pharmacokinetics;
Hippocampus/*metabolism/*pathology/surgery;
Male;
Mesenchymal Stem Cell Transplantation/methods;
Radiopharmaceuticals/pharmacokinetics;
Rats;
Rats, Sprague-Dawley;
Reproducibility of Results;
Sensitivity and Specificity;
Tissue Distribution;
Treatment Outcome
- From:Journal of Korean Medical Science
2015;30(9):1232-1240
- CountryRepublic of Korea
- Language:English
-
Abstract:
Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) may be a promising modality for treating medial temporal lobe epilepsy. 18F-fluorodeoxyglucose positron emission tomography (FDG-PET) is a noninvasive method for monitoring in vivo glucose metabolism. We evaluated the efficacy of hUCB-MSCs transplantation in chronic epileptic rats using FDG-PET. Rats with recurrent seizures were randomly assigned into three groups: the stem cell treatment (SCT) group received hUCB-MSCs transplantation into the right hippocampus, the sham control (ShC) group received same procedure with saline, and the positive control (PC) group consisted of treatment-negative epileptic rats. Normal rats received hUCB-MSCs transplantation acted as the negative control (NC). FDG-PET was performed at pre-treatment baseline and 1- and 8-week posttreatment. Hippocampal volume was evaluated and histological examination was done. In the SCT group, bilateral hippocampi at 8-week after transplantation showed significantly higher glucose metabolism (0.990 +/- 0.032) than the ShC (0.873 +/- 0.087; P < 0.001) and PC groups (0.858 +/- 0.093; P < 0.001). Histological examination resulted that the transplanted hUCB-MSCs survived in the ipsilateral hippocampus and migrated to the contralateral hippocampus but did not differentiate. In spite of successful engraftment, seizure frequency among the groups was not significantly different. Transplanted hUCB-MSCs can engraft and migrate, thereby partially restoring bilateral hippocampal glucose metabolism. The results suggest encouraging effect of hUCB-MSCs on restoring epileptic networks.
