Age-related NADPH Oxidase (arNOX) Activity Correlated with Cartilage Degradation and Bony Changes in Age-related Osteoarthritis.
10.3346/jkms.2015.30.9.1246
- Author:
Min Jung KIM
1
;
Hyun Je KIM
;
Young Hoon HONG
;
Choong Ki LEE
;
Yong Woon KIM
;
Oog Jin SHON
;
In Hwan SONG
Author Information
1. Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu, Korea. yhhong@med.yu.ac.kr
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
ENOX1 Protein, Human;
Age-related NOX;
Aging;
Osteoarthritis;
Cartilage
- MeSH:
Biomarkers/metabolism;
Cartilage Diseases/*enzymology;
Cartilage, Articular/*enzymology;
Enzyme Activation;
Female;
Humans;
Male;
Middle Aged;
NADH, NADPH Oxidoreductases;
Osteoarthritis, Knee/*diagnosis/*enzymology;
Osteoporosis/*diagnosis/*enzymology;
Reproducibility of Results;
Sensitivity and Specificity;
Statistics as Topic
- From:Journal of Korean Medical Science
2015;30(9):1246-1252
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to investigate the age-related NADPH oxidase (arNOX) activity in patients with age-related knee osteoarthritis (OA). Serum and cartilage arNOX activities were determined using an oxidized ferricytochrome C reduction assay. Full-thickness knee joint cartilages obtained through total knee replacement surgery were graded according to the Outerbridge (OB) classification. Radiographic severity of OA was determined on Knee X-rays according to the Kellgren-Lawrence (K/L) grading system. Cartilage beta-galactosidase, HIF-1alpha, and GLUT-1 expression levels were evaluated as markers for tissue senescence, hypoxia, and glycolysis. Higher arNOX activities occurred with higher levels of cartilage beta-galactosidase, HIF-1alpha, and GLUT-1 (P = 0.002). arNOX activity in cartilages with surface defects (OB grade II, III) was higher than in those without the defects (OB grade 0, I) (P = 0.012). Cartilage arNOX activity showed a positive correlation with serum arNOX activity (r = -0.577, P = 0.023). Serum arNOX activity was significantly higher in the OA subgroup with bilateral ROA than in the OA with no or unilateral ROA (2.449 +/- 0.81, 2.022 +/- 0.251 nM/mL, respectively, P = 0.019). The results of this study demonstrate that OA itself is not a cause to increase arNOX activities, however, arNOX hyperactivity is related to a high degree of cartilage degradation, and a high grade and extent of ROA in age-related OA.
