Development of microporous calcium phosphate coated nerve conduit for peripheral nerve repair.
- Author:
Jong Ho LEE
;
Soon Jeong HWANG
;
Won Jae CHOI
;
Soung Min KIM
;
Nam Yeol KIM
;
Kang Min AHN
;
Hoon MYUNG
;
Byoung Moo SEO
;
Jin Young CHOI
;
Pill Hoon CHOUNG
;
Myung Jin KIM
;
Hyun Man KIM
;
Kyung Pyo PARK
;
Joong Soo KIM
- Publication Type:Original Article
- Keywords:
Nerve regeneration;
Milipore conduits;
Schwann cells;
Calcium phosphate coating
- MeSH:
Axons;
Calcium*;
Cell Proliferation;
Cell Survival;
Crystallins;
Fibroblasts;
Membranes;
Nerve Regeneration;
Peripheral Nerves*;
Regeneration;
Schwann Cells
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2003;29(3):151-156
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed to develop a useful nerve conduit which provides favorable environment for Schwann cell viability and proliferation. Milipore membrane of 0.45um pore size was selected because it permits nutritional inflow from the outside of the conduit and prevents from invading the fibrotic tissue into the conduit. The membrane was rolled and sealed to form a conduit of 2mm diameter and 20mm length. To improve the axonal regeneration and to render better environment for endogenous and exogenous Schwann cell behaviour, the microgeometry and surface of conduit was modified by coating with thin film of calcium phosphate. Cellular viability within the conduit and attachment to its wall were assessed with MTT assay and SEM study. Milipore filter conduit showed significantly higher rate of Schwann cell attachment and viability than the culture dish. However, the reverse was true in case of fibroblast. Coating with thin film of low crystalline calcium phosphate made more favorable environment for both cells with minimal change of pore size. These findings means the porous calcium phosphate coated milipore nerve conduit can provide much favorable environment for endogenous Schwann cell proliferation and exogenous ones, which are filled within the conduit for the more advanced strategy of peripheral nerve regeneration, with potential of reducing fibrotic tissue production.
