Noninvasive monitoring of mouse renal allograft rejection using micro-CT.
10.4174/astr.2015.88.5.276
- Author:
Jiangang HOU
1
;
Masayuki FUJINO
;
Songjie CAI
;
Qiang DING
;
Xiao Kang LI
Author Information
1. Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.
- Publication Type:Original Article
- Keywords:
Kidney transplant;
Micro-CT;
Noninvasive monitoring
- MeSH:
Allografts*;
Animals;
Biopsy;
Follow-Up Studies;
Graft Rejection;
Kidney;
Kidney Transplantation;
Mice*;
Transplants
- From:Annals of Surgical Treatment and Research
2015;88(5):276-280
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Acute renal graft rejection can only be definitively diagnosed by renal biopsy. However, biopsies carry a risk of renal transplant injury and loss. Micro-CT is widely used in preclinical studies of small animals. Here, we propose micro-CT could noninvasively monitor and evaluate renal location and function in a mouse kidney transplant model. METHODS: Orthotopic kidney transplantation was performed in a BALB/c -to- C57BL/6j or C57BL/6j-to- C57BL/6j mouse model. After optimizing imaging techniques, five mice were imaged with micro-CT and the findings were verified histologically. RESULTS: Micro-CT can monitor and evaluate renal location and function after orthotopic kidney transplantation. There were no mice deaths while renal transplants were failure. CONCLUSION: We propose that graft micro-CT imaging is a new option that is noninvasive and specific, and can aid in early detection and follow-up of acute renal rejection. This method is potentially useful to improve posttransplant rejection monitoring.
