Virulence Characteristics of Sucrose-fermenting Vibrio vulnificus Strains.
10.3343/kjlm.2010.30.5.507
- Author:
Seong Jung KIM
1
;
Choon Mee KIM
;
Sung Heui SHIN
Author Information
1. Department of Emergency Medicine, Chosun University Medical School, Gwangju, Korea.
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Vibrio vulnificus;
Sucrose;
Thiosulfate-citrate-bile salts-sucrose agar;
PCR;
rDNA sequencing
- MeSH:
Animals;
Bacterial Proteins/genetics;
Fermentation;
Mice;
RNA, Ribosomal, 16S/genetics;
Sequence Analysis, DNA;
Sucrose/*metabolism;
Vibrio vulnificus/genetics/growth & development/*pathogenicity;
Virulence
- From:The Korean Journal of Laboratory Medicine
2010;30(5):507-510
- CountryRepublic of Korea
- Language:English
-
Abstract:
We identified 6 sucrose-fermenting Vibrio vulnificus strains and examined their virulence characteristics. They were all encapsulated, motile, capable of producing toxins and utilizing transferrin-bound iron, cytotoxic to cultured cells, and virulent enough to kill mice. They could be definitely identified only by genetic identification methods such as PCR, and not by conventional culture-based identification methods such as API 20E (bioMerieux, France). These results indicate that it is essential to adopt genetic approaches as early as possible in order to avoid misdiagnosis of such strains, especially in clinical situations.
