Distinct Mutation of p53 Gene in Bladder Cancer of Smoking and Nonsmoking Patients.
- Author:
Hei Young SHIM
1
;
Yeong Cheol HEO
;
Youl Hee CHO
Author Information
1. Department of Urology, Inha University College of Medicine, Sungnam, Korea.
- Publication Type:Original Article
- Keywords:
p53 mutation;
Smoking;
Transitional cell carcinoma;
Bladder;
PCR-SSCP
- MeSH:
Axons;
Carcinogenesis;
Carcinoma, Transitional Cell;
Exons;
Genes, p53*;
Genes, Tumor Suppressor;
Humans;
Smoke*;
Smoking*;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:Korean Journal of Urology
1998;39(3):247-250
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: It is well known that smoking is one of the most important etiologic factor in bladder cancer and mutations of p53 tumor suppressor gene are the important step in carcinogenesis of urinary bladder. In this study, we investigated the difference in pattern and rate of p53 gene mutation between smoker and non-smoker MATERIALS AND METHODS: Of 26 bladder transitional cell carcinoma, 16 cases were smoker and 10 cases were non-smoker. We evaluated mutation of the p53 gene concentrated on axon 5 through 8, using polymerase chain reaction- single strand conformation polymorphism(PCR-SSCP) with radioisotope. RESULTS: 3 cases(18.7%) of 16 smoker were found to have p53 gene mutation, but none of 10 non-smoker was found. 2 of 3 cases of p53 gene mutation were found in exon 5 and 1 in exon 7. The pattern of p53 gene mutation was different in 3 cases. CONCLUSIONS: Although the more cases will be needed in this study, we think that a mutation of p53 in bladder cancer may be associated with cigarette smoking.
