Mutation of Ha-ras Oncogene in Rat Salivary Gland Tumors Induced by DMBA.
- Author:
Byung Il YEH
1
;
Dong Pyou HAN
;
Joon Hyung SOHN
;
Joon Ho YOON
;
Hye An LEE
;
Sei Jin CHANG
;
In Deok KONG
;
Hyun Won KIM
;
Jong Whan CHOI
Author Information
1. Department of Biochemistry, Institute of Basic Medical Science, Yonsei University Wonju College of Medicine, Wonju, Korea. biochoi@wonju.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Salivary gland tumor;
Ha-ras oncogene;
7,12-dimethylbenz[a]anthracene
- MeSH:
9,10-Dimethyl-1,2-benzanthracene*;
Animals;
Codon;
DNA;
Genes, ras*;
Head;
Incidence;
Neck;
NIH 3T3 Cells;
Oncogenes;
Point Mutation;
Rats*;
Salivary Glands*
- From:Cancer Research and Treatment
2001;33(6):495-499
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The incidence of salivary gland tumor is approximately 2% among all head and neck tumors, of which malignant cases account for only about 5%. Much research has been performed in order to clarify the mechanism of oncogene activation, however salivary gland tumors remain understudied. We performed this study in order to characterize the ras gene in these tumors. MATERIALS AND METHODS: We treated white rats with 7, 12-dimethylbenz[a]anthracene (DMBA) and confirmed the occurrence of salivary gland tumors after ten to thirty weeks. Isolated genomic DNAs from tumor tissues were added to NIH 3T3 cells. In order to detect Ha-ras mutations, we performed a two-step PCR-RFLP and 7analyzed the mutated sequences. RESULTS: We induced salivary gland tumors by DMBA treatment in white rats. Isolated DNAs from the tumor tissues transformed the NIH 3T3 cells. Point mutations were observed in codons 12 and 61 of the Ha-ras oncogene. The total frequency of point mutations was 13.9% in DMBA-induced salivary gland tumors in rats. CONCLUSION: Our results demonstrate that a variety of cancers ras oncogene mutations were also found in salivary gland tumors. We confirmed that a point mutation of the Ha-ras oncogene in a DMBA-induced salivary gland tumor occurs at a frequency of 13.9%.
