The Effect of Intrathecal Bupivacaine with Hypothermia on Neuronal Protection against Transient Spinal Cord Ischemia in Rats.
10.4097/kjae.2006.51.2.207
- Author:
Jeong Rak LEE
1
;
Jeong Gil LEEM
;
Seung Jun HWANG
;
Dong Min JANG
;
Jung Won KIM
Author Information
1. Department of Anesthesiology and Pain Medicine, College of Medicine, Inje university, Ilsan, Korea.
- Publication Type:Original Article
- Keywords:
bupivacaine;
hypothermia;
spinal cord ischemia
- MeSH:
Anesthetics, Local;
Animals;
Aorta, Thoracic;
Bupivacaine*;
Catheters;
Cell Death;
HSP70 Heat-Shock Proteins;
Humans;
Hypothermia*;
Inflation, Economic;
Ischemia;
Male;
Models, Animal;
Neurons*;
Neuroprotective Agents;
Rats*;
Rats, Sprague-Dawley;
Reperfusion;
Spinal Cord Ischemia*;
Spinal Cord*
- From:Korean Journal of Anesthesiology
2006;51(2):207-215
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Local anesthetics can reduece excitotoxic neuronal injury from ischemia. We investigated neuroprotective effects of intrathecally administered bupivacaine and hypothermia in rat model of transient spinal cord ischemia. METHODS: A PE-10 intrathecal catheter was implanted into thirty six male Sprague-Dawley rats through L4-5 interlaminar space. Animals of normothermia (N) and hypothermia (H) groups were administered 15microliter of normal saline, and 15microliter of 0.5% bupivacaine for bupivacaine (B) and bupivacaine-hypothermia (BH) groups. Transient spinal cord ischemia was induced by inflation of a 2 F Fogarty catheter placed into aortic arch for 12 minutes. During ischemia, rectal temperature was maintained to 37.0+/-0.5 degrees C for N and B groups, 34.5 +/- 0.5degrees C for H and BH groups. Motor and sensory deficit score were assessed 2 and 24 hour after reperfusion. Lumbar spinal cords were harvested for histopathology, and for immunoreactivity of heat shock protein 70 (HSP70). RESULTS: The motor and sensory deficit score of N and B group was significantly higher than H group (P < 0.05) and BH group (P < 0.05). There were also significant difference in the motor and sensory deficit score between H and BH group at 24 hr (P < 0.05). Neuronal cell death and immunoreactivity of HSP70 was frequently observed in the N and B groups, but not in the H and BH groups. CONCLUSIONS: These results suggest that intrathecal bupivacaine did not provide neuroprotection during normothermic transient spinal cord ischemia in rats, but it can enhance neuroprotective effects of hypothermia.
