Involvement of Nuclear Factor Kappa B in High-Fat Diet-Related Pancreatic Fibrosis in Rats.
- Author:
Ming Xian YAN
1
;
Hong Bo REN
;
Yi KOU
;
Min MENG
;
Yan Qing LI
Author Information
1. Department of Gastroenterology, Shandong Qianfoshan Hospital, Shandong University School of Medicine, Shandong, China.
- Publication Type:Original Article
- Keywords:
High-fat diet;
Pancreatic fibrosis;
NF-kappa B;
Intercellular adhesion molecule 1;
Tumor necrosis factor-alpha
- MeSH:
Animals;
Blotting, Western;
Diet, High-Fat;
Fibrosis;
Gene Expression;
Humans;
Inflammation;
Intercellular Adhesion Molecule-1;
Male;
NF-kappa B;
Pancreas;
Pancreatic Stellate Cells;
Rats;
Rats, Wistar;
Tumor Necrosis Factor-alpha
- From:Gut and Liver
2012;6(3):381-387
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: High-fat diets contribute to pancreatic fibrogenesis, but the pathogenesis remains unclear. This study investigated the role of nuclear factor kappa B (NF-kappaB) in high-fat diet-induced pancreatic fibrosis in rats. METHODS: Male Wistar rats were fed a high-fat diet or standard normal chow for 20 weeks. Pancreatic fibrosis was determined by Sirius red staining. Immunohistochemical staining, reverse transcription-polymerase chain reaction and Western blotting were used to identify NF-kappaB-associated genes or protein expressions. RESULTS: Inflammation, fat deposition, pancreatic stellate cell activation and fibrosis were observed in the pancreases of the high-fat diet group. NF-kappaB subunit p65 (NF-kappaB/p65) expression was localized to the nucleus, and intercellular adhesion molecule 1 (ICAM-1) was over-expressed. Pancreatic gene expression levels of NF-kappaB/p65, ICAM-1 and tumor necrosis factor alpha were all elevated significantly in rats fed a high-fat diet compared with control rats. Western blotting also revealed significantly increased levels of ICAM-1 and nuclear NF-kappaB/p65 in rats fed high-fat diets comparison with control rats. CONCLUSIONS: NF-kappaB is involved in high-fat diet-related pancreatic fibrosis.
