Expression of Lectin like Oxidized Low Density Lipoprotein Receptor-1 in the Spontaneous Hypertensive Rat with High Cholesterol Diet.
10.5646/jksh.2011.17.2.57
- Author:
Ki Seok KIM
1
;
No Kwan PARK
;
Song Yi KIM
;
Dong Woon KIM
;
Seung Jae JOO
;
Myeong Chan CHO
Author Information
1. Department of Internal Medicine, Jeju National University School of Medicine, Jeju, Korea. kiseok@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
Atherosclerosis;
Hypertension;
Oxidative stress
- MeSH:
Animals;
Aorta, Thoracic;
Atherosclerosis;
Blood Pressure;
Blotting, Western;
Cholesterol;
Cholesterol, LDL;
Diet;
Eosine Yellowish-(YS);
Glycosaminoglycans;
Hematoxylin;
Hypercholesterolemia;
Hypertension;
Immunohistochemistry;
Lipoproteins;
Lipoproteins, LDL;
Oxidation-Reduction;
Oxidative Stress;
Plasma;
Rats;
Rats, Inbred SHR
- From:Journal of the Korean Society of Hypertension
2011;17(2):57-64
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Lectin-like, oxidized, low-density lipoprotein receptorreceptors (LOX-1) recognizes recognize vascular oxidized low-density lipoprotein (LDL), which may play an important role in the pathogenesis of atherosclerosis. We investigated the expressions expression of LOX-1 and redox-regulating thyoredoxinthioredoxin systems in a hypertension and hypercholesterolemia rat model. METHODS: Spontaneously hypertensive rats (SHR) and Wistar-Kyoto rat (WKY) rats were fed with a normal cholesterol diet (NC) and a high cholesterol diet (HC) for 4 weeks. Plasma LDL cholesterol levels and blood pressure were measured at 1 and 4 weeks. Histological changes of atherosclerosis in the vessel was evaluated by hematoxylin and eosin staining and immunocytochemistry. The expressions expression of LOX-1 and thyoredoixnthioredoxin were measured by Western western blot analysis. RESULTS: In the SHR groupsgroup, blood pressure after 4 weeks was significantly higher than initial levels. LDL-cholesterol levels in the SHR-HC group were increased at 4 weeks (15.3 +/- 2.6 mg/dL vs. 20.2 +/- 2.6 mg/dL, p < 0.01) compared with the SHR-NC group. In oxyblot analysis, the degree of oxidative stress of in the SHR-HC group was significantly higher than in the SHR-NC group (p < 0.05). The expressions expression of LOX-1 and Trx were was significantly increased in the SHR-HC group compared with the SHR-NC group (p < 0.05) on western blot analysis. Focal overexpressions overexpression of LOX-1 were was observed at the intima layer of the thoracic aorta, and was which wereonly observed in the SHR-HC group. CONCLUSIONS: The expressions expression of LOX-1 and oxidative stress were was significantly increased in the "hypertension with hypercholesterol" rat model. These findings suggested suggest that LOX-1 and redox systems may play a certain role in development and progression of atherosclerosis.
