Detection of RTP801, a Gene That is Differentially Expressed in Irradiated HeLa Cells.
- Author:
Young Sook LEE
1
;
Moon June CHO
;
Jeung Hoon LEE
;
Woong Hee LEE
;
Jun Sang KIM
Author Information
1. Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Korea.
- Publication Type:Original Article
- Keywords:
RTP801;
Radiation;
HeLa cells;
Real time PCR
- MeSH:
Blotting, Northern;
Clone Cells;
Down-Regulation;
Genes, vif*;
HeLa Cells*;
Humans;
Radiotherapy;
Real-Time Polymerase Chain Reaction;
RNA;
RNA, Messenger
- From:Cancer Research and Treatment
2004;36(4):263-270
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To quantify the effects of irradiation on the expression levels of a differentially expressed gene, RTP801, in HeLa cells. MATERIALS AND METHODS: Total RNA was isolated from irradiated and non-irradiated HeLa cells. A subtraction library was constructed, from which 88 random clones were screened. The expression patterns of one clone, detected by reverse Northern blotting, were quantified by real time RT-PCR, using CYBR green I dye. RESULTS: RTP801, a hypoxia-inducible factor-I-responsive gene, was identified as a differentially expressed gene in HeLa cells exposed to X-ray. Real time RT-PCR showed that the mRNA levels of RTP801 were greatly diminished by radiation. CONCLUSIONS: These results suggest that down-regulation of hypoxia-inducible factor-I-responsive genes, such as RTP801, in irradiated HeLa cells may result in reductions in the radiotherapy resistance of tumor cells.
