The variation of thiodiglycolic acid concentration in urine with vinyl chloride exposure levels in rats.
- Author:
Seung Jun WANG
1
;
Bong Suk CHA
;
Jae Hoon ROH
;
Dong Chun SHIN
;
Myung Su KIM
;
Keun Jae JEON
Author Information
1. Department of Preventive Medicine and Institute of Occupational Medicine, Wonju College of Medicine Yonsei University, Korea.
- Publication Type:Original Article
- Keywords:
Vinyl chloride monomer;
Thiodiglycolic acid
- MeSH:
Animals;
Diazomethane;
Methylation;
Rats*;
Vinyl Chloride*
- From:Korean Journal of Occupational and Environmental Medicine
1999;11(4):431-438
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: It is the objective of this research to identify the variation of thiodiglycolic acid (TdGA) in urine with vinyl chloride monomer (VCM) exposure levels through methylation. METHODS: After rats were exposed to vinyl chloride monomer of 4 levels, 0 mg/m3, 50 mg /m3, 150 mg/m3, 500 mg/m3, respectively, of which urine was sampled in each sampling time of 0 hour, 2 hours, 4 hours, 8 hours, 16 hours, 24 hours, 36 hours, 48 hours. After urine had been methylated with diazomethane in the preliminary experiment and the peak of 146 m/z had been verified, the main experiment was done. RESULTS: In the variation of TdGA with sampling times, concentration of TdGA increased rapidly in 4 hours and then decreased after 8 hours. When the variation of urinary .TdGA concentration in urine according to exposure level of VCM was verified through Kruskal-Wallis statistical method at each sampling time, the significant increment with the exposure levels at 2 hours, 4 hours, 8 hours after exposure was clarified. CONCLUSION: TdGA concentration in urine with increment of VCM exposure level increased, especially significantly at 2 hours, 4 hours, 8 hours of sampling time.
