Analysis of porcine macrophage immune response to antigenic molecules and short chain fatty acids.
10.12729/jbr.2015.16.2.047
- Author:
Na Eun HAN
1
;
Eun Joo LEE
;
Kwan Sik PARK
;
In Sook JEON
;
Hak Kyo LEE
;
Ki Duk SONG
;
Joong Kook CHOI
Author Information
1. Division of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 362-763, Korea. jkchoi@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
LPS;
macrophage;
immune response;
DNA microarray;
short chain fatty acid
- MeSH:
Acetylmuramyl-Alanyl-Isoglutamine;
Animals;
Animals, Domestic;
Antigen Presentation;
Biomarkers;
Butyrates;
Clone Cells;
Cytokines;
Energy Metabolism;
Fatty Acids*;
Gene Expression Regulation;
Genome;
Hand;
Homicide;
Humans;
Macrophages*;
Membrane Proteins;
Mice;
Microbiota;
Monocytes;
Oligonucleotide Array Sequence Analysis;
Peptides;
Phagocytosis;
Signal Transduction;
Sus scrofa;
Toll-Like Receptor 4
- From:Journal of Biomedical Research
2015;16(2):47-52
- CountryRepublic of Korea
- Language:English
-
Abstract:
Macrophages play an important role in both the innate and adaptive immune responses. These include phagocytosis, killing of microorganisms, antigen presentation, and induction of immune cytokines and antimicrobial genes. Macrophage activity is reported to be controlled by diverse exogenous antigenic or endogenous metabolic molecules, and the underlying mechanisms are well documented in human and mouse macrophage cells. Bacterial lipopolysaccharide (LPS) is known to be one of the most potent stimuli activating macrophages through the toll like receptor 4 (TLR4) signaling pathway. There are other antigenic molecules, such as muramyl dipeptide (MDP) and outer membrane protein A (OmpA), that are also known to activate immune cells. On the other hand, short chain fatty acids (SCFAs) such as acetate and butyrate are produced by gut microbiota and control host energy metabolism and signal transduction through GPR receptors. However, there are few studies demonstrating the effects of these molecules in macrophages from domestic animals, including domestic pigs. In this study, we attempted to characterize gene expression regulation in porcine macrophages (PoM2, Pig Monocytes clone 2) following treatment with LPS, MDP, OmpA, and two short chain fatty acids using porcine genome microarray and RT-PCR techniques. A number of novel porcine genes, including anti-microbial peptides and others, appeared to be regulated at the transcriptional level. Our study reports novel biomarkers such as SLC37A2, TMEN184C, and LEAP2 that are involved in the porcine immune response to bacterial antigen LPS and two short chain fatty acids.
