Chemopreventive effect of alpha-viniferin in azoxymethane-induced mouse colorectal tumor and Caco-2 cells.
10.12729/jbr.2015.16.2.060
- Author:
Dong Hoon KWAK
1
;
Sang Kyung SHIN
;
So Young YOUM
;
Tae Wang KIM
;
Youngsoo KIM
;
Byeongwoo AHN
Author Information
1. College of Veterinary Medicine, Chungbuk National University, Cheongju 362-763, Korea. bwahn@cbu.ac.kr
- Publication Type:Original Article
- Keywords:
alpha-viniferin;
Caco-2 cell;
chemoprevention;
colorectal cancer;
mouse
- MeSH:
Aberrant Crypt Foci;
Animals;
Azoxymethane;
Caco-2 Cells*;
Carcinogenesis;
Caspase 3;
Cell Count;
Chemoprevention;
Colonic Neoplasms;
Colorectal Neoplasms*;
Cyclooxygenase 2;
Dextrans;
Drinking Water;
Fluorescent Antibody Technique;
Humans;
In Situ Nick-End Labeling;
Mice*;
Nitric Oxide Synthase Type II;
Rodentia;
Sodium
- From:Journal of Biomedical Research
2015;16(2):60-66
- CountryRepublic of Korea
- Language:English
-
Abstract:
alpha-Viniferin (AVF), a trimer of resveratrol, is known to have an anti-inflammatory effect via inhibition of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). It has been reported that up-regulated COX-2 and iNOS are expressed in colon cancer tissues of humans and rodents as well as pre-neoplastic aberrant crypt foci (ACF) of rodents. In this study, chemopreventive effects of AVF were assessed in Caco-2 cells as well as azoxymethane (AOM)-induced colorectal tumorigenesis in mice. Anti-tumor effect of AVF with regards to apoptotic induction was assessed by TUNEL and caspase-3 expression in human colon cancer Caco-2 cells. For development of ACF, AOM was administered with to mice intraperitoneally at a dose of 10 mg/kg once a week for 3 weeks. To induce colitis-related colon cancer, mice were administered a single dose of AOM (10 mg/kg) and 2% dextran sodium sulfate in drinking water. Mice treated with 0.05 and/or 0.1 mg of AVF by gavage showed significantly reduced development of ACF and colorectal tumors. Immunofluorescence detection in Caco-2 cells showed reduced COX-2 and iNOS expression, whereas cleavage of caspase-3 and apoptotic cell numbers increased upon AVF treatment. Immunostaining showed reduced expression levels of COX-2 and iNOS expression along with increased cleaved caspase-3 expression increased upon AVF treatment. These results suggest that AVF has chemopreventive effects on colorectal cancer via anti-inflammatory potential and pro-apoptotic activity.
