Identification of Two Novel NPM1 Mutations in Patients with Acute Myeloid Leukemia.
- Author:
Yongbum JEON
1
;
Sang Won SEO
;
Seonyang PARK
;
Seungman PARK
;
So Yeon KIM
;
Eun Kyung RA
;
Sung Sup PARK
;
Moon Woo SEONG
Author Information
- Publication Type:Original Article
- Keywords: NPM1; Nucleophosmin; AML
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Amino Acid Motifs; Base Sequence; DNA Mutational Analysis; Exons; Female; Humans; Leukemia, Myeloid, Acute/*genetics/pathology; Male; Middle Aged; Mutation; Nuclear Proteins/*genetics; Young Adult
- From:Annals of Laboratory Medicine 2013;33(1):60-64
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Genetic abnormalities in adult AML are caused most frequently by somatic mutations in exon 12 of the NPM1 gene, which is observed in approximately 35% of AML patients and up to 60% of patients with cytogenetically normal AML (CN-AML). METHODS: We performed mutational analysis, including fragment analysis and direct sequencing of exon 12 of the NPM1 gene, on 83 AML patients to characterize the NPM1 mutations completely. RESULTS: In this study, NPM1 mutations were identified in 19 (22.9%) of the 83 AML patients and in 12 (42.9%) of the 28 CN-AML patients. Among the 19 patients with NPM1 mutations, type A NPM1 mutations were identified in 16 (84.2%) patients, whereas non-A type NPM1 mutations were observed in 3 (15.8%) patients. Two of the 3 non-A type NPM1 mutations were novel: c.867_868insAAAC and c.869_873indelCTTTAGCCC. These 2 novel mutant proteins display a nuclear export signal motif (L-xxx-L-xx-V-x-L) less frequently and exhibit a mutation at tryptophan 290 that disrupts the nucleolar localization signal. CONCLUSIONS: This study suggests that novel NPM1 mutations may be non-rare and that supplementary sequence analysis is needed along with conventional targeted mutational analysis to detect non-A types of NPM1 mutations.
