Behavioral and Histochemical Changes in MPTP-treated C57BL/6 Mice: A Model for Parkinson's Disease.
- Author:
Jae Woo KIM
1
;
Sang Kun LEE
;
Kyung Min HA
Author Information
1. Department of Neurology, College of Medicine, Dong-A University, Korea.
- Publication Type:Original Article
- MeSH:
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine;
Animals;
Locus Coeruleus;
Mice*;
Models, Animal;
Motor Activity;
Parkinson Disease*;
Substantia Nigra;
Tyrosine 3-Monooxygenase;
Ventral Tegmental Area
- From:Journal of the Korean Neurological Association
1993;11(2):175-180
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The effects of 1-methyl-4-phenyl-1, 2. 3, 6-tetrahydropyridine (MPTP) on behavioral and histochemical changes were investigated in C57BL/6 mice. For immunocytochemis try, one group of mice received a dose of 2X30mg/kg of MPTP given 12hours apart and the other group 30mg/kg/day of MPTP for 7days. Locomotor activit,v was measured during 120minutes after a single injection of 30mg/kg of MPTP. We compared the numbers of tyrosine hydroxylase (TH)-positive cell bodies using immunocytochemical technique in the substantia nigra, ventral tegmental area and locus ceruleus 10 days after the last injection of MPTP. There was a significant decrease in locomotor activity during 100minutes after injection of MPTP and the number of TH-positive cell bodies in the substantia nigra of the mice which received the dose of 30mg/kg/day for 7 days, but not in the ventral tegmental area or the locus ceruleus. But 30mg/kg of MPTP given 12 hours apart failed to produce a significant decrease in the number of TH-positive cell bodies in any three catecholamine nuclei examined. It is concluded that MPTP-treated C57BL/6 mice provide a useful model for studying characteristics of Parkinson's disease and the dose of 30mg/kg/day for 7 days is more effective in the animal model for Parkinson s disease in C57BL/6 mice.
