Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection.
- Author:
Kiju KIM
1
;
Dooree KIM
;
Jisun SUN
;
Soyeon PARK
;
Youngjae CHO
;
Hyun Jeong KO
;
Hong Gu JOO
;
Tae Wook HAHN
Author Information
1. College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea. twhahn@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
humoral immunity;
mucosal immunity;
Salmonella
- MeSH:
Animals;
Antibodies;
Enzyme-Linked Immunosorbent Assay;
Female;
Humans;
Immunity, Humoral;
Immunity, Mucosal;
Immunoglobulin G;
Mice;
Salmonella;
Salmonella enterica;
Salmonella typhimurium;
Sprains and Strains
- From:Korean Journal of Veterinary Research
2013;53(2):95-101
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The study was carried out to evaluate and compare the immune responses in mice experimentally infected with either wild-type or isogenic mutants of Salmonella enterica serovars Enteritidis (SE), Salmonella Typhimurium (ST) and Gallinarum (SG). The mutant strains were constructed by allelic replacement of some virulence-associated genes in the wild-type strains. Seven-week-old female BALB/c mice were orally or intraperitoneally inoculated by injecting bacterial suspension. To evaluate the immune responses, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT) assay were conducted with serum and fecal samples. As a result, the mice group infected orally with the SE mutant strain showed the highest level of specific IgA-secreting splenocytes, compared to the other groups. The peritoneally injected groups showed the greater levels of IgG1 than the orally injected groups, which was in a good agreement with the previous studies. In addition, the mutant infected groups had the similar secretion levels of antibodies with the wild-type infected groups. These results demonstrated that the SE mutant strain elicited humoral immune response as much as wild-type, implying that it can be useful as a delivery vehicle as well as a candidate of a live attenuated vaccine.
