Polymorphism in Codons 10 and 25 of the Transforming Growth Factor-beta1 Gene in Korean Population and in Patients with Liver Cirrhosis and Hepatocellular Carcinoma.
- Author:
Oh Sang KWON
1
;
Suk Ho SONG
;
Ki Tak JU
;
Moon Gi CHUNG
;
Dong Kyun PARK
;
Sun Suk KIM
;
Yeon Suk KIM
;
Yang Suh KOO
;
Yu Kyung KIM
;
Duck Joo CHOI
;
Ju Hyun KIM
;
You Jin HWANG
;
Kwan Soo BYUN
;
Chang Hong LEE
Author Information
1. Department of Internal Medicine, Gachon Medical School, Inchon, Korea. kos@ghil.com
- Publication Type:Original Article ; English Abstract
- Keywords:
Transforming growth factor beta;
Liver cirrhosis;
Carcinoma;
hepatocellular;
Polymorphism (genetic)
- MeSH:
Adult;
Aged;
Carcinoma, Hepatocellular/*genetics;
Codon/genetics;
Female;
Genotype;
Humans;
Korea;
Liver Cirrhosis/*genetics;
Liver Neoplasms/*genetics;
Male;
Middle Aged;
*Polymorphism, Genetic;
Sequence Analysis, Protein;
Transforming Growth Factor beta/*genetics;
Transforming Growth Factor beta1
- From:The Korean Journal of Gastroenterology
2003;42(3):212-219
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND/AIMS: The genetic polymorphism of transforming growth factor-beta1 (TGF-beta1) at codons 10 and 25 which influences the production of TGF-beta1 is related to fibrogenesis in the lung and liver. We evaluated the genetic polymorphism at codons 10 and 25 in controls and in patients with liver cirrhosis (LC) and hepatocellular carcinoma (HCC). METHODS: Blood samples were collected from controls (n=35), patients with LC (n=64), and HCC (n=49). Genomic DNA was isolated and polymerase chain reaction (PCR) was done for a segment including codons 10 and 25. The results of direct sequencing for PCR products were compared between the controls and the patients. RESULTS: There was no genetic polymorphism at codon 25 and three types of genetic polymorphism at codon 10. The leucine homozygous genotype (CTG/CTG) at codon 10 was more common in patients with LC than the controls (p=0.01) and especially in patients with LC caused by HBV (p=0.004). The polymorphism at codons 10 in patients with HCC was similar to the controls. However, leucine homozygous genotype was more common in patients with HCC of uninodular morphology than those of massive morphology (p=0.007). CONCLUSIONS: The genetic polymorphism of TGF-beta1 at codon 10 might be associated with LC and morphology of HCC. The potential usefulness of TGF-beta1 genotyping needs further studies in large scale.
