Ascorbic Acid Enhances Nitric Oxide Production in Trabecular Meshwork Cells.
10.3341/kjo.2005.19.3.227
- Author:
Jae Woo KIM
1
Author Information
1. Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Ascorbic acid;
Hydrogen peroxide;
Nitric oxide;
Trabecular meshwork cell
- MeSH:
Trabecular Meshwork/cytology/*drug effects/*metabolism;
Nitric Oxide/*biosynthesis;
Humans;
Dose-Response Relationship, Drug;
Cells, Cultured;
Cell Survival/drug effects;
Ascorbic Acid/administration & dosage/*pharmacology
- From:Korean Journal of Ophthalmology
2005;19(3):227-232
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study investigated the role of ascorbic acid on the production of nitric oxide (NO) in the trabecular meshwork (TM) cells. METHODS: After primarily cultured human TM cells were exposed to 1, 10, and 100 micrometer of L-ascorbic acid (LAA), with or without co-administration of 1 mM sodium nitroprusside or 100 micrometer hydrogen peroxide for 48 hr, cellular survival and NO production were measured with MTT and Griess assay, respectively. RESULTS: LAA significantly potentiated NO production in a dose-dependent manner (p< 0.05) without affecting cell viability. LAA increased cell viability after hydrogen peroxide-induced oxidative stress in a dose-dependent manner. LAA enhanced NO production in TM cells and showed a cytoprotective effect against hydrogen peroxide-induced oxidative stress. CONCLUSIONS: LAA might be involved in the regulation of trabecular outflow by enhancing NO production in TM cells.
