Expression of TLR-4 in epithelial serous ovarian cancer.
- Author:
Chel Hun CHOI
1
;
Jeong Won LEE
;
Jung Joo CHOI
;
Woo Young KIM
;
Tae Joong KIM
;
Je Ho LEE
;
Byoung Gie KIM
;
Duk Soo BAE
Author Information
1. Department of Obstetrics & Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. ds123.bae@samsung.com
- Publication Type:Original Article
- Keywords:
Toll-like receptor 4;
Ovarian serous adenocarcinoma
- MeSH:
Adenocarcinoma;
Cytoplasm;
Immunohistochemistry;
Neoplasm Metastasis;
Ovarian Neoplasms*;
RNA;
RNA, Messenger;
Toll-Like Receptor 4
- From:Korean Journal of Gynecologic Oncology
2007;18(3):180-186
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: This study was to determine the expression of Toll-like receptor 4 (TLR-4) in ovarian serous adenocarcinoma tissues. METHODS: TLR-4 expression was evaluated at the RNA level by real-time quantitative RT-PCR, in 24 fresh frozen ovarian serous adenocarcinoma tissues and 9 normal ovarian tissues. TLR-4 expression was also evaluated by immunohistochemistry (IHC) in each three ovarian carcinoma tissues and normal ovarian tissues. RESULTS: Positive immunoreactivity for TLR-4 was observed in the normal ovarian tissues but not in the ovarian carcinoma tissues. The staining was localized in the cytoplasm as well as on the cell surface. Real-time quantitative RT-PCR revealed that TLR-4 expression was significantly lower in tumors than in normal ovarian tissues (p=0.0003). There were no significant correlations between clinical parameters and the expression level of TLR-4 mRNA in ovarian serous adenocarcinomas. However, tumors without LN metastasis (p=0.068) and lower grade (p=0.075) showed trends of higher TLR-4 mRNA expression. CONCLUSION: TLR-4 expression was significantly lower in ovarian serous adenocarcinoma tissues than in normal ovarian tissues, and further studies on TLR-4 signaling pathway in ovarian carcinoma are needed.
