Growth inhibition and induction of apoptosis in cervical cancer cell lines by green tea polyphenols.
- Author:
Ying Min JIN
1
;
Sang Lyun NAM
;
Woong Sik AHN
Author Information
1. Department of Oncology, Harbin Red Cross Central Hospital, Harbin, China.
- Publication Type:Original Article
- Keywords:
Green tea polyphenols;
Cervical cancer;
Apoptosis;
Chemoprevention
- MeSH:
Apoptosis*;
Catechin;
Cell Line*;
Chemoprevention;
DNA Fragmentation;
Guanosine Triphosphate;
Humans;
Inhibitory Concentration 50;
Neutral Red;
Polyphenols*;
Tea*;
Trypan Blue;
Uterine Cervical Neoplasms*
- From:Korean Journal of Obstetrics and Gynecology
2002;45(4):560-568
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The purpose of this article is to estimate the anti-cancer effects of the major components of the green tea (polyphenols, catechin and EGCG) and the mechanism of EGCG on different cervical cancer cell lines. METHODS: Six cervical cancer cell lines (HeLa, HeLaS3, Caski, SiHa, HT3 and C33A) were treated with 20 microgram/ml green tea polyphenols (GTPs), 50 micrometer catechin and various concentrations of (-)-epigallo- catechin-3-gallate (EGCG). The viabilities were determined by trypan blue exclusion assay, neutral red assay and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. DNA fragmentation and nuclear condensation were used to see whether EGCG-induced anti-proliferation effect was due to apoptosis. RESULTS: Both GTPs, catechin and EGCG had growth inhibition effects on cervical cancer cell lines, but EGCG appeared to be the most effective. What's more, the sensitivity of each cell lines to EGCG was different. HT3 cells (HPV negative, mutant type p53) were most sensitive to EGCG (estimate IC50: 10 micrometer). Caski (HPV-16 positive, wild type p53) and HeLaS3 cells (HPV-18 positive, wild type p53) were less sensitive (estimate IC50: 35 and 70 micrometer respectively). EGCG-induced apoptosis can be seen in all the cell lines and it happened as early as 8 hours after EGCG treatment. CONCLUSION: Green tea or EGCG alone will be beneficial to the cervical cancer patients.
