Increase of Angiogenesis and Neurogenesis, and Neurobehavioral Recovery by Transplanted Mesenchymal Stem Cells in the Ischemic Rat Brain.
- Author:
Woo Sung LEE
1
;
Pil Woo HUH
Author Information
1. Department of Biomedical Science, 1Department of Neurosurgery, Graduate School, The Catholic University of Korea, Korea.
- Publication Type:Original Article
- Keywords:
Mesenchymal stem cells;
Cb.infraction;
Angiogenesis;
Neurogenesis
- MeSH:
Animals;
Bone Marrow;
Brain Ischemia;
Brain*;
Infarction, Middle Cerebral Artery;
Mesenchymal Stromal Cells*;
Mice;
Models, Animal;
Neurogenesis*;
Rats*;
Stem Cells;
Transplants
- From:Korean Journal of Cerebrovascular Surgery
2007;9(4):277-285
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECT: In this study, murine mesechymal stem cells (MSC) was grafted to cerebral ischemia rats, the differentiation of the transplanted MSC was assessed, and at that time, the association of the level of the recovery of neurological function. METHODS: As experimental animals, the transient middle cerebral artery occlusion (MCAo) rat model was used, and MSC collected from the bone marrow of C57/Bl6 mice were cultured, 5microliter(105 microliter) cells marked with the fluorescent substance 3.3'-dilinoleyloxacarbocyanine(DIO) were transplanted into the ischemic cortex. Angiogenesis and neurogenesis were examined and the neurobehavioral evaluation were done. To assess the differentiation level of MSC, and it was evaluated as the percentage of the mean area of stained cells within each region of interest (ROI). RESULTS: In the MCA transplanted group, Neurobehavioral score was improved to twelve points one hour, one day, and three days after transplant, and seven days and fourteen days after transplant, to eleven points and nine points, respectively. The result of immunohistochemical staining showed that the angiogenesis on the day seven was 3.15% and on the day fourteen was 4.26%, the neurogenesis on the day seven was 2.29% and on the day fourteen was 3.82%. CONCLUSION: On the day seven and fourteen after MCS transplantation, the phenomenon of the actively ongoing angiogenesis and neurogenesis was detected, and it was found that at that time, the recovery of neurological function occurred. However, the level of differentiation from MSC was small, and thus it was not statistically significant.
