The Duffy Blood Group Genotypes in Asian Populations.
- Author:
Chae Seung LIM
1
;
Young Kee KIM
;
Kap No LEE
Author Information
1. Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea. malarim@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Duffy;
Asian;
PCR-RFLP
- MeSH:
Asian Continental Ancestry Group*;
Bangladesh;
Blood Group Antigens;
Digestion;
DNA;
Ethidium;
Ethnic Groups;
Gels;
Genotype*;
Human Migration;
Humans;
Phenotype;
Polymerase Chain Reaction;
Sepharose
- From:Korean Journal of Blood Transfusion
2007;18(3):145-151
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Duffy phenotypes vary widely among different ethnic groups and populations from distinct locations. Several assays for genotyping Duffy blood groups have recently been developed, and PCR-restriction fragment length polymorphism (RFLP) is the most widely used method for human Duffy blood group typing. In this study, we employed PCR-RFLP and analyzed the genotypes of Duffy blood groups among five Asian populations including a Korean population. METHODS: Two-hundred-fifty-four peripheral blood samples were collected from different Asian populations. PCR was performed by using primers designed for the amplification of the GATA and FYAB regions of the Duffy gene. The restriction enzymes Sty I and Ban I were used for the digestion of PCR products and the DNA products were separated on agarose or polyacrylamide gels stained with ethidium bromide. RESULTS: The genotype frequencies of FY*A/FY*B, FY*A/FY*A, FY*B/FY*B, FYnull in the Korean population were 14.2%, 84.9%, 1%, 0% respectively. The frequency of the Duffy genotype of Thai, Chinese and Pilipino populations were similar to that of the Korean population. However, a population from Bangladesh had a high FY*B frequency (43.4%) as compared to the other Asian populations. CONCLUSION: Among the five Asian populations analyzed, the Duffy genotype for a Bangladesh population is different from the East Asian populations. This finding may reflect a history of human migration of ancient Asian populations.
