Inhibition of Cyclooxygenase-2 (Cox-2) Expression by Genistein in Breast Cancer Cell-line.
10.4048/jkbcs.2003.6.4.277
- Author:
Hyuck SONG
1
;
Tae Young KOO
;
Ji Hoon PARK
;
Ki Hak SONG
;
Sung Tae KIM
;
In Seok CHOI
;
Won Joon CHOI
;
Woo Kyun MOK
;
Hyun Sik MIN
;
Dae Sung YOON
Author Information
1. Department of Surgery, Konyang University School of Medicine, Daejeon, Korea. dsyoonmd@kyuh.co.kr
- Publication Type:Original Article
- Keywords:
Breast carcinoma;
Cox-2inhibitor;
Genistein
- MeSH:
Asian Continental Ancestry Group;
Breast Neoplasms*;
Breast*;
Cyclooxygenase 2*;
Diet;
Female;
Genistein*;
Humans;
Incidence;
Isoflavones;
MCF-7 Cells;
RNA, Messenger
- From:Journal of Korean Breast Cancer Society
2003;6(4):277-282
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The isoflavones in soy are likely to contribute to the historically low incidence of breast cancer among Asian women that consume traditional diets. A possible role of isoflavones in controlling the expression of cyclooxygenase-2 (Cox-2) has not previously been explored. In this study, the ability of the isoflavone, genistein, to regulate the expression of Cox-2 in breast cancer cells was evaluated. METHODS: The effects of genistein and NS-398, a Cox-2 inhibitor, were examined on the expression of Cox-2 at the mRNA level using the MDA-MB 231, and MCF-7 breast cancer cell-lines. RESULTS: In the MCF-7 cells, the Cox-2 mRNA could not be detected using RT-PCR. In the MDA-MB-231 cells, NS-398 and genistein were found to inhibit the Cox-2 mRNA expressions by 50 and 35~40%, respectively. CONCLUSION: These studies suggest that dietary isoflavonoids may contribute to the prevention or inhibition of breast cancer by inhibiting the Cox-2 expression.
