Modulation of Antibody Responses against Gnathostoma spinigerum in Mice Immunized with Crude Antigen Formulated in CpG Oligonucleotide and Montanide ISA720.
10.3347/kjp.2013.51.6.637
- Author:
Pewpan M INTAPAN
1
;
Chakrit HIRUNPETCHARAT
;
Churairat KULARBKAEW
;
Wiboonchai YUTANAWIBOONCHAI
;
Penchom JANWAN
;
Wanchai MALEEWONG
Author Information
1. Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. pewpan@kku.ac.th
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Gnathostoma spinigerum;
CpG oligonucleotide;
Th1-type response;
Th2-type response;
nematode;
immunity
- MeSH:
Adjuvants, Immunologic/*administration & dosage;
Animals;
Antibodies, Helminth/*blood;
Antigens, Helminth/*administration & dosage/*immunology;
Gnathostoma/*immunology;
Immunoglobulin G/blood;
Immunoglobulin M/blood;
Male;
Mannitol/administration & dosage/*analogs & derivatives;
Mice;
Oleic Acids/*administration & dosage;
Oligodeoxyribonucleotides/*administration & dosage;
Th1 Cells/immunology;
Th2 Cells/immunology
- From:The Korean Journal of Parasitology
2013;51(6):637-644
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response.
