Morphological and Molecular Characterization of Lymnaeid Snails and Their Potential Role in Transmission of Fasciola spp. in Vietnam.
10.3347/kjp.2013.51.6.657
- Author:
Bui Thi DUNG
1
;
Pham Ngoc DOANH
;
Dang Tat THE
;
Ho Thi LOAN
;
Bertrand LOSSON
;
Yannick CARON
Author Information
1. Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Vietnam. dung_parasitologist@yahoo.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Fasciola;
Austropeplea viridis;
Radix auricularia;
Radix rubiginosa;
Vietnam
- MeSH:
Animals;
Cluster Analysis;
DNA, Ribosomal Spacer/chemistry/genetics;
Fasciola/*isolation & purification;
Lymnaea/*anatomy & histology/genetics/*parasitology;
Molecular Sequence Data;
Phylogeny;
Sequence Analysis, DNA;
Sequence Homology, Nucleic Acid;
Vietnam
- From:The Korean Journal of Parasitology
2013;51(6):657-662
- CountryRepublic of Korea
- Language:English
-
Abstract:
Freshwater snails of the family Lymnaeidae play an important role in the transmission of fascioliasis worldwide. In Vietnam, 2 common lymnaeid species, Lymnaea swinhoei and Lymnaea viridis, can be recognized on the basis of morphology, and a third species, Lymnaea sp., is known to exist. Recent studies have raised controversy about their role in transmission of Fasciola spp. because of confusion in identification of the snail hosts. The aim of this study is, therefore, to clarify the identities of lymnaeid snails in Vietnam by a combination of morphological and molecular approaches. The molecular analyses using the second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA clearly showed that lymnaeids in Vietnam include 3 species, Austropeplea viridis (morphologically identified as L. viridis), Radix auricularia (morphologically identified as L. swinhoei) and Radix rubiginosa (morphologically identified as Lymnaea sp.). R. rubiginosa is a new record for Vietnam. Among them, only A. viridis was found to be infected with Fasciola spp. These results provide a new insight into lymnaeid snails in Vietnam. Identification of lymnaeid snails in Vietnam and their role in the liver fluke transmission should be further investigated.
