Saccharomyces boulardii Activates Expression of Peroxisome Proliferator-activated Receptor-gamma in HT-29 Cells.
- Author:
Sang Kil LEE
1
;
Hyo Jong KIM
;
Sung Gil CHI
;
Jae Young JANG
;
Ki Deok NAM
;
Nam Hoon KIM
;
Kwang Ro JOO
;
Seok Ho DONG
;
Byung Ho KIM
;
Young Woon CHANG
;
Joung Il LEE
;
Rin CHANG
Author Information
1. Departments of Internal Medicine, Kyung Hee University College of Medicine, Seoul, Korea. hjkim@khmc.or.kr
- Publication Type:Original Article ; English Abstract
- Keywords:
Saccharomyces boulardii;
PPAR-gamma
- MeSH:
Cell Proliferation;
Colon/*metabolism;
*Gene Expression;
HT29 Cells;
Humans;
Interleukin-1/metabolism;
Interleukin-8/metabolism;
Lipopolysaccharides/pharmacology;
PPAR gamma/genetics/*metabolism;
Saccharomyces/*physiology
- From:The Korean Journal of Gastroenterology
2005;45(5):328-334
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND/AIMS: Saccharomyces boulardii (S. boulardii) has been reported to be beneficial in the treatment of inflammatory bowel disease, however, little is known about its mechanism of action. Peroxisome proliferator- activated receptor-gamma (PPAR-gamma) is recently found to regulate inflammation in intestinal epithelial cells. We hypothesized that the anti-inflammatory effects of S. boulardii are mediated, in part, through PPAR-gamma. To test this hypothesis, we examined the ability of S. boulardii to modulate the expression of PPAR-gamma in human colon cells. METHODS: Effects of S. boulardii on survival and proliferation of HT-29 human colon cells were assessed by MTT and [3H]thymidine incorporation assays. PPAR-gamma expression was assessed by Western blot and RT-PCR. Induction of interleukin-8 (IL-8) expression by tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), or lipopolysaccharide (LPS) was assessed by RT-PCR. RESULTS: S. boulardii did not affect viability and proliferation of HT-29 cells. S. boulardii up-regulated PPAR-gamma expression at both mRNA and protein levels. Pretreatment of HT-29 cells with S. boulardii blocked PPAR-gamma down-regulation by TNF-alpha, IL-1beta, or LPS, whereas it ameliorated IL-8 response to these proinflammatory factors. CONCLUSIONS: S. boulardii stimulates PPAR-gamma expression and reduces response of human colon cells to proinflammatory cytokines.
