Role of nitric oxide and interleukin-1beta on expression of matrix metalloproteinase-2 in TL cell line.
- Author:
Jae Dong LEE
1
;
Jong Chul SHIN
;
Dong Eun YANG
;
Hee Bong MOON
;
Jee Hyun LEE
;
Hyun Young AHN
;
Gui SeRa LEE
;
Sa Jin KIM
;
Su Pyung KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Catholic University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Trophoblast;
Matrix metalloproteinase-2;
Nitric oxide;
Inteleukin-1beta
- MeSH:
Blotting, Western;
Cell Line*;
Gelatin;
Interleukin-1beta*;
Matrix Metalloproteinase 2*;
Nitric Oxide*;
Placenta;
RNA;
RNA, Messenger;
S-Nitroso-N-Acetylpenicillamine;
Trophoblasts
- From:Korean Journal of Obstetrics and Gynecology
2002;45(12):2225-2230
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: We studied to investigate whether nitric oxide (NO) and IL-1beta modulate MMP-2 and MMP-9 using TL cell line obtained from the normal term placenta. METHODS: After culturing TL cell line for 4 hours, we treated 0.1 mM of SNAP (NO donor) and 50 ng/ml of IL-1beta for 0, 1, 3, 6, and 12 hours, for investigating changes from time. We treated SNAP of 0, 0.01, 0.1, and 0.5 mM for 12 hours and IL-1beta of 0, 1, 10, and 50 ng/ml, for investigating changes from concentration. After extraction of total RNA, we performed reverse transcriptase-polymerase chain reaction (RT-PCR), gelatine zymography and Western blot analysis, for investigating expression of MMP-2 and MMP-9. RESULTS: MMP-9 was not observed in TL cell line. The expressions of MMP-2 mRNA and protein were gradually increased according to the culture time in SNAP treated group. The expressions of MMP-2 mRNA and protein were gradually increased according to the culture time in IL-1beta treated group. The expression of MMP-2 protein was not more increase in SNAP/IL-1beta-treated group than in IL-1beta treated group. The expression of MMP-2 protein was more reduced in SNAP/hemoglobin treated group than in SNAP treated group. MMP-2 protein activity was only increase in SNAP treated group. CONCLUSION: These results indicate that NO, rather than IL-1beta, upregulates the MMP-2 in TL cell line and furthermore may influence in the invasive process of trophoblasts.
